| Determination of drought tolerance using root activities in Robinia pseudoacada 'Idaho' transformed with mtl-D gene |
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| 作者姓名： | Wang Hua-fang Zhu Yi-hong Sun Hai-jun College of Biological Sciences and Biotechnology Beijing Forestry University Beijing P. R. China Center of Modern Education Beijing Sport University Beijing P. R. China |
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| 作者单位： | Wang Hua-fang Zhu Yi-hong Sun Hai-jun College of Biological Sciences and Biotechnology，Beijing Forestry University，Beijing 100083，P. R. China Center of Modern Education，Beijing Sport University，Beijing 100084，P. R. China |
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| 摘 要： | Idaho locust (Robinia pseudoacacia 'Idaho') is an exotic multi-purpose tree used in landscaping, soil and water conservation, fodder sources and others. To improve its drought tolerance for reclaiming arid land, five lines of transformed mtl-D gene, as osmotic regulator in plant cells, have been selected and managed to determine their drought tolerance under experimental conditions. Qualitative and quantitative variables of transformed plants were studied. The critical value of drought tolerance was determined by detecting the 2,3,5-triphenyl tetrazolium chloride (TTC) reductants in roots and soil water content (SWC). The critical value for drought tolerance was SWC 6% while for the control plants the critical SWC was 8%; a moderate level of SWC is 13% and the highest SWC for plant endurance was 18%. The method proved to be reliable and sensitive in the evaluation of drought tolerance for forest trees.
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Determination of drought tolerance using root activities in Robinia pseudoacada 'Idaho' transformed with mtl-D gene |
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| Wang Hua-fang Zhu Yi-hong Sun Hai-jun College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing ,P. R. China Center of Modern Education,Beijing Sport University,Beijing ,P. R. China.Determination of drought tolerance using root activities in Robinia pseudoacada ''''Idaho'''' transformed with mtl-D gene[J].Forestry Studies in China,2006(4). |
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| Authors: | Wang Hua-fang Zhu Yi-hong Sun Hai-jun College of Biological Sciences and Biotechnology Beijing Forestry University Beijing P R China Center of Modern Education Beijing Sport University Beijing P R China |
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| Institution: | Wang Hua-fang Zhu Yi-hong Sun Hai-jun College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,P. R. China Center of Modern Education,Beijing Sport University,Beijing 100084,P. R. China |
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| Abstract: | Idaho locust (Robinia pseudoacacia 'Idaho') is an exotic multi-purpose tree used in landscaping, soil and water conservation, fodder sources and others. To improve its drought tolerance for reclaiming arid land, five lines of transformed mtl-D gene, as osmotic regulator in plant cells, have been selected and managed to determine their drought tolerance under experimental conditions. Qualitative and quantitative variables of transformed plants were studied. The critical value of drought tolerance was determined by detecting the 2,3,5-triphenyl tetrazolium chloride (TTC) reductants in roots and soil water content (SWC). The critical value for drought tolerance was SWC 6% while for the control plants the critical SWC was 8%; a moderate level of SWC is 13% and the highest SWC for plant endurance was 18%. The method proved to be reliable and sensitive in the evaluation of drought tolerance for forest trees. |
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| Keywords: | Robinia pseudoacacia 'Idaho' root activity critical value of drought tolerance soil water content 2 3 5-triphenyl tetrazolium chloride (TTC) |
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