| 不同培养条件和卵母细胞来源对黄牛卵母细胞 第一极体排出的影响 |
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| 引用本文： | 李东伟,章美玲,张运海,章孝荣.不同培养条件和卵母细胞来源对黄牛卵母细胞 第一极体排出的影响[J].安徽农业大学学报,2013,40(4):523. |
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| 作者姓名： | 李东伟 章美玲 张运海 章孝荣 |
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| 作者单位： | 安徽农业大学动物科技学院，合肥 230036; 阜阳师范学院生命科学学院，阜阳 236037; 安徽地方畜禽遗传资源保护与生物育种省级实验室，合肥 230036; 安徽省羊繁育工程技术研究中心，合肥 230036,安徽农业大学动物科技学院，合肥 230036; 安徽地方畜禽遗传资源保护与生物育种省级实验室，合肥 230036; 安徽省羊繁育工程技术研究中心，合肥 230036,安徽农业大学动物科技学院，合肥 230036; 安徽地方畜禽遗传资源保护与生物育种省级实验室，合肥 230036; 安徽省羊繁育工程技术研究中心，合肥 230036,安徽农业大学动物科技学院，合肥 230036; 安徽地方畜禽遗传资源保护与生物育种省级实验室，合肥 230036; 安徽省羊繁育工程技术研究中心，合肥 230036 |
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| 基金项目： | 国家自然科学基金项目(31272442)资助。 |
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| 摘 要： | 研究了培养液组分浓度、培养方法和卵母细胞来源对黄牛卵母细胞第一极体（PB1）排出的影响。从屠宰场收集黄牛卵巢，使用抽吸法获取卵丘卵母细胞复合物（COCs）进行体外培养。结果表明，采用以下培养条件能够显著促进黄牛卵母细胞PB1排出：在培养液中添加10 μg·mL-1 FSH和LH、1.0 μg·mL-1 17-β E2、33.0 μg·mL-1丙酮酸钠；培养时间24 h，培养密度每50 μL培养液培养10~20枚COCs；卵巢储运温度25～37 ℃，储运时间<3 h，卵泡直径3～6 mm，卵丘细胞包裹2层以上。选择最佳的培养液组分浓度、培养方法和卵母细胞来源可获得最大PB1排出量，为下一步利用PB1进行保护物种、扩大优良母畜遗传资源的来源和植入前遗传学诊断等研究提供参考。
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| 关 键 词： | 黄牛 卵母细胞 第一极体（PB1） 体外培养 |
Effects of different culture and oocyte conditions on the first polar body extrusion of cattle oocytes |
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| LI Dong-wei,ZHANG Mei-ling,ZHANG Yun-hai and ZHANG Xiao-rong.Effects of different culture and oocyte conditions on the first polar body extrusion of cattle oocytes[J].Journal of Anhui Agricultural University,2013,40(4):523. |
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| Authors: | LI Dong-wei ZHANG Mei-ling ZHANG Yun-hai and ZHANG Xiao-rong |
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| Institution: | School of Animal Sciences and Technology, Anhui Agricultural University, Hefei 230036; School of Life Sciences, Fuyang Teachers College, Fuyang 236037; Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Hefei 230036; Engineering Research Center of Reproduction and Breeding in Sheep of Anhui Province, Hefei 230036,School of Animal Sciences and Technology, Anhui Agricultural University, Hefei 230036; Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Hefei 230036; Engineering Research Center of Reproduction and Breeding in Sheep of Anhui Province, Hefei 230036,School of Animal Sciences and Technology, Anhui Agricultural University, Hefei 230036; Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Hefei 230036; Engineering Research Center of Reproduction and Breeding in Sheep of Anhui Province, Hefei 230036 and School of Animal Sciences and Technology, Anhui Agricultural University, Hefei 230036; Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Hefei 230036; Engineering Research Center of Reproduction and Breeding in Sheep of Anhui Province, Hefei 230036 |
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| Abstract: | |
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| Keywords: | cattle oocyte polar body 1 (PB1) in vitro culture |
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