川芎嗪纳米脂质体对HUVEC细胞VEGF基因 表达的抑制作用

制备川芎嗪纳米脂质体，并对其进行质量控制。3批川芎嗪脂质体的颗粒小于220 nm；Zeta电位大于30 mV；包封率分别为43.89%±3.88％，61.23%±4.71％和84.35%±6.67％。采用MTT 和Real-time PCR方法研究川芎嗪及其脂质体对人脐静脉内皮细胞（HUVEC）增殖及对其VEGF基因表达的影响。结果表明,川芎嗪及其脂质体对HUVEC细胞的增值无统计学差异,但下调VEGF₁₆₅诱导的HUVEC细胞中VEGF基因的表达。它们可能通过抑制VEGF基因的表达而抑制血管新生，其中川芎嗪脂质体的抑制作用较川芎嗪弱，且随着包封率的提高，作用依次减弱，表明脂质体是一种缓慢释放的优良剂型，有进一步临床应用价值。

Inhibitation of tetramethylpyrazine-loaded liposomes on VEGF gene expression in HUVEC cells

The three batches of tetramethylpyrazine-loaded liposomes were prepared, and the properties ware as follows: the size of liposome was below 220 nm; Zeta electric potential was greater than 30 mV; the entrapment rates of them were 43.89%±3.88%, 61.23%±4.71% and 84.35%±6.67%, respectively. The inhibitation of tetramethylpyrazine and tetramethylpyrazine-loaded liposomes on VEGF gene in HUVEC cells were explored. The results proved that tetramethylpyrazine and tetramethylpyrazine-loaded liposomes had no effect on the proliferation of HUVEC, but regulated lower VEGF gene expression and inhibited angiogenesis. Tetramethylpyrazine- loaded liposomes had lower effect than tetramethylpyrazine. Furthermore, the effects would be weakened with the increase of entrapment rate. It illustrat that the liposome is a novel preparation as the drug delivery for the slow release. It would be wide perspective for the application into the clinic in the future.