| 猪PLIN1基因单核苷酸多态检测与遗传分析 |
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| 引用本文： | 黄龙,余大华,何小雷,朱卫华,孟云,薛玮玮,丁月云,殷宗俊.猪PLIN1基因单核苷酸多态检测与遗传分析[J].安徽农业大学学报,2013,40(5):701. |
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| 作者姓名： | 黄龙 余大华 何小雷 朱卫华 孟云 薛玮玮 丁月云 殷宗俊 |
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| 作者单位： | 安徽农业大学动物科技学院，安徽地方畜禽遗传资源保护与生物育种省级实验室，合肥 230036,安徽安泰农业集团，广德 242200,安徽农业大学动物科技学院，安徽地方畜禽遗传资源保护与生物育种省级实验室，合肥 230036,安徽农业大学动物科技学院，安徽地方畜禽遗传资源保护与生物育种省级实验室，合肥 230036,安徽农业大学动物科技学院，安徽地方畜禽遗传资源保护与生物育种省级实验室，合肥 230036,安徽农业大学动物科技学院，安徽地方畜禽遗传资源保护与生物育种省级实验室，合肥 230036,安徽农业大学动物科技学院，安徽地方畜禽遗传资源保护与生物育种省级实验室，合肥 230036,安徽农业大学动物科技学院，安徽地方畜禽遗传资源保护与生物育种省级实验室，合肥 230036 |
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| 基金项目： | 国家自然科学基金（31171200）, 科技富民强县专项行动计划, 国家农业科技成果转化资金（2011GB2C300017）, 省级农业科技专家大院（2012）和科技部星火计划重点项目（2010GA710001）共同资助。 |
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| 摘 要： | 以皖南黑猪、安庆六白猪、圩猪和霍寿黑猪共236头猪为研究对象，采用PCR-SSCP方法检测猪脂滴包被蛋白（Perilipin, PLIN1）基因的遗传多态性。结果表明，在皖南黑猪、安庆六白猪PLIN1基因3 519 bp碱基处发生A>G突变，检测到AA、AB、BB 3种基因型, 其中AA基因型频率在这2个群体中分别为8.97%和29.23%，AB基因型频率分别为43.59%和56.92%，BB基因型频率分别为47.44% 和13.85%；皖南黑猪、安庆六白猪的多态信息含量（PIC）分别为0.3353和0.3690，在该位点均处于中度多态；卡方检验表明皖南黑猪群体和安庆六白猪群体均处于哈迪-温伯格平衡状态(P>0.05)；在圩猪、霍寿黑猪群体中PLIN1基因的第2外显子没有检测到突变。PLIN1基因的第3外显子在4个群体中均没有检测到突变。
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| 关 键 词： | 猪 PLIN1基因 单核苷酸多态性 遗传分析 |
Single nucleotide polymorphism detection and genetic analysis of porcine PLIN1 gene |
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| HUANG Long,YU Da-hu,HE Xiao-lei,ZHU Wei-hu,MENG Yun,XUE Wei-wei,DING Yue-yun and YIN Zong-jun.Single nucleotide polymorphism detection and genetic analysis of porcine PLIN1 gene[J].Journal of Anhui Agricultural University,2013,40(5):701. |
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| Authors: | HUANG Long YU Da-hu HE Xiao-lei ZHU Wei-hu MENG Yun XUE Wei-wei DING Yue-yun and YIN Zong-jun |
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| Institution: | Anhui?Provincial?Key?Laboratory?of?Local?Livestock?and?Poultry?Genetic?Resources?Conservation?and?Bio-Breeding, School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,Anhui Antai Agriculture Group, Guangde 242200,Anhui?Provincial?Key?Laboratory?of?Local?Livestock?and?Poultry?Genetic?Resources?Conservation?and?Bio-Breeding, School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,Anhui?Provincial?Key?Laboratory?of?Local?Livestock?and?Poultry?Genetic?Resources?Conservation?and?Bio-Breeding, School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,Anhui?Provincial?Key?Laboratory?of?Local?Livestock?and?Poultry?Genetic?Resources?Conservation?and?Bio-Breeding, School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,Anhui?Provincial?Key?Laboratory?of?Local?Livestock?and?Poultry?Genetic?Resources?Conservation?and?Bio-Breeding, School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,Anhui?Provincial?Key?Laboratory?of?Local?Livestock?and?Poultry?Genetic?Resources?Conservation?and?Bio-Breeding, School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036 and Anhui?Provincial?Key?Laboratory?of?Local?Livestock?and?Poultry?Genetic?Resources?Conservation?and?Bio-Breeding, School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036 |
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| Abstract: | |
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| Keywords: | pig PLIN1 gene single nucleotide polymorphism genetic analysis |
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