河北省花生地方品种基于SSR标记的遗传多样性

 【目的】揭示河北省花生地方品种的遗传多样性,为花生育种提供理论依据。【方法】利用20对SSR引物对75个河北省不同植物类型花生地方品种遗传多样性进行分析。【结果】共检测到65个等位基因,每个位点的等位基因变幅为2～6个,平均3.25个;平均Shannon信息指数为0.5448,变幅为0.1680(7G02)～1.3617(PM15);平均Nei基因多样性指数为0.6458,变幅为0.3385(7G02)～0.9013(PM384);普通型花生地方品种的遗传多样性明显大于多粒型和珍珠豆型。采用类平均法对欧氏距离进行聚类,可以将各地方品种分为两大类,第Ⅰ类群为珍珠豆型和多粒型花生地方品种,第Ⅱ类群为普通型花生地方品种,品种间的亲缘关系与地理来源关系不大。【结论】SSR检测结果表明,河北省花生地方品种的多样性程度较高。

Genetic Diversity of Peanut Landraces in Hebei Province Revealed by SSR Markers

【Objective】 In order to assess the genetic diversity of peanut landraces from Hebei province and provide a theoretical basis for peanut breeding. 【Method】 Genetic diversity of 75 accessions of peanut landraces with different botanical types, collected from Hebei province, was evaluated by 20 SSR molecular markers. 【Result】 Sixty-five alleles were detected. The average number of alleles per locus was 3.25 with a range from 2 to 6. Total Shannon information index of per locus varied widely from 0.1680 (7G02) -1.3617 (PM15) with an average value of 0.5448; Total Nei’s genetic diversity index of Nei per locus varied widely from 0.3385 (7G02) -0.9013 (PM384) with an average value of 0.6458; The accessions of A. hypogaea showed higher genetic diversity than those of A. vulgaris and A. fastigiata. Cluster analysis with UPGMA showed that the materials tested could be divided into 2 groups: the first group was A. vulgaris and A. fastigiata group, the second was A. hypogaea group and the genetic relationship among these landraces was not in accord with their geographical origins. 【Conclusion】 The peanut landraces from Hebei province showed higher genetic diversity revealed by SSR markers.