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Grafting in vitro Regenerated Pea (Pisum sativum L.) Shoots as a Method for Obtaining Mature Plants
Authors:L. Natali   A. Cavallini
Affiliation:Dr. L. Natali;, Dr. A. Cavallini, Dipartimento di Biologia delle Piante Agrarie, Sezione di Genetica, Via Matteotti 1/B, I-56124 Pisa (Italy).
Abstract:A method of grafting pea plants is proposed to allow rapid availability of a large number of mature plants from in vitro regenerated shoots. Plant regeneration was obtained from macerated vegetative apices and immature embryos, but regenerated shoots rooted with low frequency (ca. 10 %). When those shoots were grafted onto seedling stocks of the same cultivar, 80 to 85 % of the grafts survived, grew to maturity and produced seed.
Keywords:Pisum sativum    grafting    plant regeneration
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