影响枣胚培试管苗生根因子的研究

以婆婆枣胚培试管苗为试材，研究了影响其不定根形成的一系列因子。结果表明，IBA、IAA、NAA三种生长素单独使用时以IAA2.0mg/L生根率最高，达60.7%；但IAA与IBA配合使用时有明显增效作用，最佳浓度配比为IBA1.0mg/L+IAA0.01mg/L，其生根率最高达91.0%，而6—BA抑制根的形成。1/2MS+20g/L蔗糖为基本培养基较好。另外，也探讨了苗质及暗培养对生根的影响。

Studies of affecting factors on rooting induction of embryogenic test tube seedling of Chinese Date in tissue culture

A series of factors affecting rooting were studied in embryo culture of Chinese Date(Popozao).The results of experiment are as follows: IAA was more effective than IBA and NAA in stimulating adventitious rooting regeneration, reaci hed as high as 60 7% of rooting rate when IAA is 2 0 mg/l;But,the coordinating result of IAA and IBA is better than that of IAA and IBA by single, the best compose is IBA 1 0 mg/l and IAA 0 01 mg/l,and the best medium of inducting rooting is 1/ 2 MS IBA1 0 mg/l IAA0 01 mg/l sugar 20 g,91 0% rooting rate obtained;Cytokinin BA restrain the formation of adventitious rooting; Other factors of affecting rooting, such as the quality of vitro seedling and dark time, were described, too.