| Studies on Factors Affecting the in vitro Germination and Growth of Taxus cuspidata Embryos |
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| 作者姓名： | LiuBenye YeHechun |
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| 作者单位： | [1]NortheastAgriculturalUniversity,Harbin150030,PRC [2]InstiuteofBotany,AcademiaSininca,Beijing100093,PRC |
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| 摘 要： | Factors affecting the in vitro germination and growth of Taxus cuspidata embryos were exam-ined.DCR medium was the best among 6 basal media tested;embryos from stage Ⅱ seeds were the optimal developmantal stage for in vitro germination; as the seeds approached maturity,germinability of embryos decease ,Embryos ,cultured in darkness tended to from callus and de-crease the frequency of germination;inoculation method obviously affected frequency of embryo germination,Embryos cultured in optimal conditions developed into seedilings in 2 months.
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| 关 键 词： | 东北红豆杉 体外萌发 胚胎培养 影响因素 |
Studies on Factors Affecting the in vitro Germination and Growth of Taxus cuspidata Embryos |
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| Liu Benye,Zhang Yanping.Studies on Factors Affecting the in vitro Germination and Growth of Taxus cuspidata Embryos[J].Journal of Northeast Agricultural University,1997,4(1):55-59. |
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| Authors: | Liu Benye Zhang Yanping |
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| Abstract: | Factors affecting the in vitro germination and growth of Taxus cuspidata embryos were examined. DCR medium was the best among 6 basal media tested; embryos from stage II seeds were the optimal developmental stage for in vitro germination; as the seeds approached maturity, germinability of embryos decrease. Embryos cultured in darkness tended to form callus and decrease the frequency of germination; inoculation method obviously affected frequency of embryo germination. Embryos cultured in optimal conditions developed into seedlings in 2 months. |
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| Keywords: | Taxus cuspidata embryo culture in vitro germination |
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