Implication of melatonin in oocyte maturation in Indian major carp Catla catla |
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Authors: | Saumen Kumar Maitra Asamanja Chattoraj Sharmistha Bhattacharyya |
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Institution: | (1) Department of Zoology, Visva-Bharati University, Santiniketan, 731 235, India |
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Abstract: | Importance of melatonin (N-acetyl-5-methoxytryptamine) in the regulation of oocyte maturation has been studied in a carp Catla catla. Melatonin secretory cells were immunocytochemically localized only in the end vesicle. Diurnal and seasonal studies indicated
that the serum levels of melatonin exhibit a minimum diurnal value in the mid-day of all seasons, but the peak value is attained
either at mid-night or just before the onset of light. Moreover, highest seasonal value of melatonin was noted in the post-spawning
phase. Administration of melatonin at different doses (25, 50, or 100 μg/100 g body weight) for 1, 15, or 30 days resulted
in either pro- or anti-gonadal effects depending on the reproductive seasons. In vitro study revealed that incubation of oocytes
with melatonin 4h prior to addition of MIH in the medium led to an accelerated rate of oocyte maturation through the formation
of a complex of two proteins (MPF), cyclin B and cyclin dependant kinase, Cdc2. Moreover, melatonin pre-incubation considerably
increased MIH stimulation of histone H1 phosphorylation as compared to MIH alone. Taken together, gathered information promotes
the idea of a physiological role of melatonin in the maturation of oocytes in Catla catla. |
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Keywords: | Annual cycle Catla Carp Diurnal cycle Melatonin Oocyte maturation |
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