不同基因型孔雀草高效植株再生体系的建立

 将孔雀草品种T. patula'Little Hero Golden'的叶片、子叶和下胚轴接种于4种含有不同浓度IAA、NAA和BA的MS培养基上，比较不同外植体的再生植株的能力。结果表明，叶片再生植株能力较子叶和下胚轴强。随后，进一步将其叶片接种于9种添加不同浓度NAA和BA的MS培养基上，分化结果显示，在MS+NAA 1.5 mg·L-1+BA 1.0 mg·L-1的培养基上植株再生率最高，达75%。5份材料的叶片分别接种在该培养基上，再生率均达70%以上，其中T. patula21614的再生率达85%。所有再生植株均能成苗并表现出正常的形态特征。从而建立了适合于５种基因型孔雀草的离体高效再生体系。

The Establishment of Efficient Regeneration System for Different Genotypes of Tagetes patula L.

In order to test the regeneration ability of different explants, the leaves, cotyledons and hypocotyls of T. patula'Little Hero Golden' were cultured on 4 kinds of media, MS + NAA 0.5 mg·L-1+ BA 1.0 mg·L-1, MS + NAA 1.0mg·L-1 + BA 2.0 mg·L-1, MS + NAA 1.5 mg·L-1 + BA 2.0 mg·L-1 and MS + IAA 3.0 mg·L-1 + BA 2.5 mg·L-1. The results showed that the regeneration ability of leaves was higher than that of cotyledons and hypocotyls. The leaves were then cultured on 9 kinds of MS media with different combinations of NAA and BA, and the highest regeneration frequency was obtained on MS medium supplemented with 1.5 mg·L-1NAA and 1.0 mg·L-1 BA, and the regeneration rate was up to 75%. The medium was tested for other 4 genotypes, and all the lines were successfully regenerated with frequencies from 70% to 85%. All the regenerated plantlets developed into complete plants of normal morphological characteristics.