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A Simplified Rice DNA Extraction Protocol for PCR Analysis |
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| Authors: | CHEN Wen-yue CUI Hai-rui BAO Jin-song ZHOU Xiang-sheng SHU Qing-yao |
| Institution: | 1.Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China;2. Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China;3.Zhejiang Seed Administration Station, Hangzhou 310020, China |
| Abstract: | A simple protocol was established for DNA extraction using etiolated rice seedlings, whereby rice DNA was directly extracted in 0.5 mol/L NaOH solution in a single eppendorf tube. Results of comparative PCR analyses and electrophoresis showed that the DNA extracted using this method was as good and useful as that using standard CTAB method. |
| Keywords: | DNA extraction rice polymerase chain reaction molecular marker simple sequence repeats transgene |
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