| 低氧诱导透明颤菌血红蛋白基因在大肠杆菌中的表达 |
| |
| 引用本文： | 张锋,赵晓瑜,周艳芬. 低氧诱导透明颤菌血红蛋白基因在大肠杆菌中的表达[J]. 华北农学报, 2006, 21(Z1): 20-22 |
| |
| 作者姓名： | 张锋 赵晓瑜 周艳芬 |
| |
| 作者单位： | 河北大学,生命科学学院,河北,保定,071002 |
| |
| 摘 要： | 合成透明颤菌血红蛋白基因(vgb)的天然低氧启动子,与vgb结构基因拼接得到570 bp的vgb基因,将该基因克隆到pUC18质粒中重组为pUC_LV质粒,转化大肠杆菌得到重组子。通过低氧诱导10 h后,重组菌的密度较对照增加了67.4%,经SDS_PAGE和CO差光谱分析证明重组菌表达了有活性的透明颤菌血红蛋白(VHb)。
|
| 关 键 词： | 透明颤菌血红蛋白基因 天然启动子 大肠杆菌 低氧诱导 |
| 文章编号： | 1000-7091(2006)增刊-0020-03 |
| 修稿时间： | 2006-07-20 |
Hypoxia-induced Expression of Vitreoscilla Hemoglobin Gene in Escherichia coli |
| |
| ZHANG Feng,ZHAO Xiao-yu,ZHOU Yan-fen. Hypoxia-induced Expression of Vitreoscilla Hemoglobin Gene in Escherichia coli[J]. Acta Agriculturae Boreali-Sinica, 2006, 21(Z1): 20-22 |
| |
| Authors: | ZHANG Feng ZHAO Xiao-yu ZHOU Yan-fen |
| |
| Abstract: | The 570 bp Vitreoscilla hemoglobin gene(vgb) was obtained by synthesizing the native promoter of vgb and jointing it with vgb structural gene.The recombinant vector pUC-LV constructed by cloning the gene into pUC18 plasmid was transformed into Escherichia coli.After ten hours of induction under low dissolved oxygen conditions,the density of recombinant strain increased 67.4% compaired with that of control.The results of SDS-PAGE and carbon monoxide(CO) difference spectrum analysis indicated that the recombinant strain expressed Vitreoscilla hemoglobin(VHb) having biological activity. |
| |
| Keywords: | Vitreoscilla hemoglobin gene(vgb) Native promoter Escherichia coli Hypoxia induction |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
