西洋百合“卢浮宫”的组培技术研究

以鳞片为外植体、MS为基本培养基,以不同激素成分及不同质量浓度水平下诱导西洋百合"卢浮宫"的小鳞茎状突起,进行组培试验。结果表明:"卢浮宫"百合的诱导以取其完整的鳞片为外植体、并以鳞片背部突起处紧贴于MS+6-BA2.0mg·L-1+NAA0.2mg·L-1的培养基上诱导效果为最好。"卢浮宫"百合的诱导是由外植体(鳞片)产生小鳞茎状突起而分化成苗的,并不经愈伤组织分化产生。筛选出的最佳继代增殖培养基为MS+6-BA0.7mg·L-1+IBA0.3mg·L-1,生根培养基为1.2MS+6-BA0.3mg·L-1+IBA0 7mg·L-1。移植基质选择河泥和珍珠岩(体积比7∶3)混合,成活率达98%。

Study on Tissue Culture of Lilium japonicum cv.'Louvre'

Using bulb scale as the explant and MS as the basic media, the bulbous projection of Lilium japonicum cv.'Louvre'was induced and cultured with different hormones in differet concentration levels. The result revealed that the best way to induce 'Louvre'was to use its whole scale, whose back was stuck on the medium that contains 2.0 mg·L-1 of 6-BA, 0.2 mg·L-1 of NAA and MS as the explant.The induction of 'Louvre'scale was to produce the bulbous projection which differentiated into seedings, but not to produce it from callus induction. MS containg 0.7 mg·L-1 of 6-BA and 0.3 mg·L-1 of IBA was optimized as the best medium for shoot-tip culture and subculture, and 1/2MS containing 0.3 mg·L-1 of 6-BA and 0.7 mg·L-1 of IBA was optimized as the best medium for rooting culture. The material for transplanting was the combanation of river mud and pearly rock(cubic comparison is 7∶3). The survival rate of Lilium shoots could reach 98%.