弯杆菌噬菌体的分离及其转导试验 |
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引用本文: | 沈正达,奥格.弯杆菌噬菌体的分离及其转导试验[J].甘肃农业大学学报,1986(4). |
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作者姓名: | 沈正达 奥格 |
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作者单位: | 中国甘肃农业大学,美国科州州立大学 |
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摘 要: | 从溶原性胎儿弯杆菌性病亚种(Campylobacter fetus subspVenerealis)分得了一株温和型噬菌体,证明具有转导抗生素(头胞金素和链霉素)抗性决定簇至敏感受体细菌的能力,其中转导头胞金素的试验结果为首次报道。该噬菌体在敏感细菌生长的半固体倾注培养物上形成混浊的蚀斑,其平均直径约为0.5毫米。在电子显微镜下,噬菌体的头部呈多角形,尾纤细,其长度约为其头部的4—5倍。
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Isolation of A Transducing Bacteriophage for Campylobacter |
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Abstract: | A bacteriophage isolated from a lysogen of Campylobacter fetus subspecies venercalis was capable of transducing anti- biotic resistance determinants from antibiotic resistant donors to antibiotic-sensitive strains.The phage formed turbid plaques(about 0.5mm average diameter)on lawns of sensitive cells.Examination of phage preparations by elctron microscopy showed polyhedralshaped phage heads and slender tails approximately 4 to 5 times longer than the heads. |
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