| A3启动子缺陷piggyBac转座子在家蚕中的转基因研究 |
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| 引用本文： | 杨惠娟,庄兰芳,范伟,兰天云,丁农,陆长德,钟伯雄. A3启动子缺陷piggyBac转座子在家蚕中的转基因研究[J]. 蚕业科学, 2008, 34(1): 41-44 |
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| 作者姓名： | 杨惠娟 庄兰芳 范伟 兰天云 丁农 陆长德 钟伯雄 |
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| 作者单位： | 浙江大学动物科学学院,杭州,310029;国家分子生物学重点实验室,中国科学院上海生命科学研究院,生物化学与细胞生物学研究所,上海,200031;浙江省湖州市农业科学院,浙江湖州,313000 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家重点基础研究发展计划(973计划)，教育部高等学校博士学科点专项科研基金，浙江省科技厅科技计划 |
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| 摘 要： | 构建了A3启动子缺陷piggyBac转座质粒,以增强型绿色荧光蛋白基因EGFP为标记基因对家蚕品种N is-tari进行转基因实验,发现其具有较高的表达EGFP的转化效率,G0代中EGFP阳性蛾区检出占总注射蚕卵的比率达0.618%,且EGFP的表达呈组织特异性。PCR实验分析也证实了标记基因的成功导入。该实验中标记基因及转基因阳性个体均易于检出,为启动子缺陷转基因方法在家蚕组织特异性启动子筛选研究上的应用奠定了基础。
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| 关 键 词： | 家蚕 转基因 启动子缺陷 piggyBac转座子 |
Transgenic Research of A3 Promoter-Deficient piggy Bac-derived Transposon in the Silkworm,Bombyx mori |
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| YANG Hui-Juan,ZHUANG Lan-Fang,FAN Wei,LAN Tian-Yun,DING Nong,LU Chang-De,ZHONG Bo-Xiong. Transgenic Research of A3 Promoter-Deficient piggy Bac-derived Transposon in the Silkworm,Bombyx mori[J]. Acta Sericologica Sinica, 2008, 34(1): 41-44 |
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| Authors: | YANG Hui-Juan ZHUANG Lan-Fang FAN Wei LAN Tian-Yun DING Nong LU Chang-De ZHONG Bo-Xiong |
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| Abstract: | We constructed the plasmid harbored the A3 promoter-deficient piggyBac-derived transposon and the enhanced green fluorescence protein(EGFP) gene.The constructed plasmid was microinjected into the eggs of Nistari strain,along with the helper plasmid.Transgenic experiment results showed that the transformation efficiency was high,and the rate of number of G0 moths whose offspring expressed the EGFP to the number of eggs injected was 0.618%.Moreover,EGFP was expressed with the manner of specific tissue,and the positive moths are easy to be observed in G0 generation.The transgenic silkworms were also verified by PCR method.Our results indicated that A3 promoter-deficient transformation in the silkworm maybe used to screen the promoter activated in specific tissue. |
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| Keywords: | Bombyx mori Transgene Promoter-deficient piggyBac transposon |
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