Hemolytic complement measurement in eleven species of nonhuman primates |
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Authors: | LR Ellingsworth CA Holmberg BI Osburn |
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Institution: | 1. Department of Experimental Immunology and Pathology, California Primate Research Center, University of California, Davis, CA, U.S.A.;2. Veterinary Medicine Teaching and Research Center, 3981 South K, Tulare, CA, U.S.A.;3. Department of Veterinary Pathology, School of Veterinary Medicine, University of California, Davis, CA, U.S.A. |
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Abstract: | A microtiter system was used to measure hemolytic complement levels in serum from eleven nonhuman primate species. The species studied were (rhesus macaque), (bonnet macaque), (pig-tailed macaque), (crab-eating macaque), (stumptailed macaque), (yellow baboon), (olive baboon), (African green monkey), (owl monkey), (spider monkey), and (thick-tailed galago).The optimal hemolytic complement titer of the various nonhuman primate species was found to vary with different species sources of erythrocytes and anti-erythrocyte reagents used in the assay. No single erythrocyte and anti-erythrocyte test reagent produced optimal titers for all of the primate species examined. Sera from several species was found to have high spontaneous lytic activity towards non-sensitized sheep erythrocytes which for six species ( and ) was equal to the titer for antibody sensitized erythrocytes. Evidence of alternate pathway complement activation as a possible reason for the high titer of lytic activity towards unsensitized erythrocytes could not be demonstrated for any nonhuman primate species. In one species, , the sensitizing activity of normal serum for sheep erythrocytes was shown to be in the IgM containing fraction obtained with gel filtration and to be absorbed by boiled sheep erythrocyte stroma which contains Forssman antigen. |
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