棘孢木霉分泌蛋白和效应子的预测及分析

棘孢木霉(Trichoderma asperellum)作为一种生防菌,在植物病害的防控中具备较高的开发以及利用价值.根据棘孢木霉CBS 433.97菌株全基因组测序获得的12557个蛋白序列,利用SignalP、TargetP、TMHMM、WoLF PSORT和big-PI Predictor生物信息学软件和预测程序...

Prediction and analysis of sereted proteins and effectors of Trichoderma asperellum

Trichoderma asperellum as a biocontrol strain has high development and application value in plant pathologic diseases. According to the 12 557 proteins in the genome of T. asperellum CBS 433.97 strain, it was in order screened by SignalP, TargetP, TMHMM, WoLF PSORT and big-PI Predictor bioinformatics softwares and prediction programs. Finally, 485 secreted protein sequences with typical characteristics were obtained. A total of 137 proteins were obtained by comparing 485 secreted proteins with the carbohydrate-active enzymes database, cysteine content analysis and Pathogen-Host Interaction database analysis. The small molecule protein of less than 300 amino acid was further screened as the candidate effector protein, and 15 proteins were obtained, including one carbohydrate-binding module family protein, one carbohydrate-esterase protein, and 13 hypothetical proteins. The results provide a theoretical basis for further study on the mechanism of action of T. asperellum genes and its subsequent utilization.