氟苯尼考纳米晶在鸡体内的药动学

氟苯尼考(florfenicol,FFC)是新型动物广谱抗菌剂,抗菌效果好,广泛应用于牛、羊、猪、水产及禽类等动物细菌性疾病的防制.本试验旨在研究FFC和氟苯尼考纳米晶(florfenicol nanocrystal,FFC-NC)在鸡体内的生物利用度.采用交叉试验法,鸡用药后,在不同时间点翅下静脉采血,利用高效液相色...

Pharmacokinetics study of florfenicol nanocrystals in hens

As a broad-spectrum antibiotic for animals, florfenicol(FFC) has good antibacterial effect and is widely used in the prevention and treatment of bacterial diseases in cattle, sheep, pigs, aquatic animals, and poultry.The purpose of this study is to evaluate the bioavailability of FFC and florfenicol nanocrystals(FFC-NC) in hens.Cross-over design method was used to evaluate the bioavailability of FFC and FFC-NC in hens.After administration, blood samples were collected from wing veins of hen at different time.The concentration of FFC in plasma samples were determined by high performance liquid chromatography(HPLC).Results showed that the established HPLC chromatogram showed a stable baseline, and the plasma peak was completely separated from the florfenicol peak.Results of recovery rate and precision met the requirements, and the repeatability were good.This method was suitable for the determination of FFC concentration in hen plasma.Results of pharmacokinetic showed that compared with the FFC group, value of time to reach peak concentration(t_max) of FFC-NC group was(0.875±0.137) h, it was significantly reduced.Values of area under the concentration-time curve(AUC_0-∞) and peak concentration(C_max) of FFC-NC group were(23.957±2.338) mg/(L·h) and(8.249±0.713) mg/L,respectively, which were significantly improved.The relative bioavailability of FFC-NC group was 3.6 times higher than that of FFC group.In conclusion, the pharmacokinetic characteristics of FFC-NC are significantly improved compared with FFC.This study provides experimental evidence for the clinical use of FFC-NC.