黔北麻羊GPx3基因克隆、生物信息学分析及在不同性腺组织中的表达分析

为探究GPx3基因在产单、多羔黔北麻羊不同性腺组织中的表达规律及其蛋白质的结构与功能,本实验以黔北麻羊为研究对象,采集单、多羔黔北麻羊的下丘脑、垂体、子宫、输卵管和卵巢提取总RNA,运用qRT-PCR技术,分析GPx3基因mRNA在单、多羔黔北麻羊5个不同性腺组织中的表达水平;PCR法扩增克隆黔北麻羊GPx3基因的CD...

Cloning,Bioinformatics Analysis and Expression Analysis of GPx3 Gene in Different Gonad Tissues of Qianbei Ma Goat

In order to explore the expression of GPx3 gene in different gonad tissues of single and multiple lamb of Qianbei ma goat and the structure and function of its protein. In this study, the total RNA of hypothalamus, pituitary, uterus, fallopian tube and ovary of single and multiple lamb of Qianbei ma goat was collected. The expression levels of GPx3 gene mRNA in five different gonad tissues of single and multiple lambs of Qianbei ma goat were analyzed by qRT-PCR technology. The PCR method was used to amplify and clone CDS region of GPx3 gene of Qianbei ma goat. The structure and function of GPx3 protein in Qianbei ma goat were analyzed and predicted by bioinformatics. The results of qRT-PCR showed that GPx3 gene was expressed in five different gonad tissues of single and multiple lamb of Qianbei ma goat, and the expression in ovary was the highest. Except for hypothalamus, the expression levels of other tissues in the multiple-lambing group were extremely higher than that in the single-lambing group. The results of T cloning showed that the CDS region sequence of GPx3 gene of Qianbei ma goat was 681 bp, and the homology with mRNA sequence of goat registered on NCBI was 99.85%. Bioinformatics analysis revealed that GPx3 protein of Qianbei ma goat might be located outside the cells and have signal peptides, which belongs to unstable hydrophilic secretory protein, and its tertiary structure is mainly composed of irregular curl and α-helix.The genetic tree analysis showed that the genetic distance of GPx3 gene from Qianbei ma goat was the closest to that of sheep, and the genetic distance was the farthest from that of chicken. In this study, GPx3 gene was cloned and analyzed by bioinformatics, and the expression differences of GPx3 gene in different gonad tissues of Qianbei ma goat were revealed,which provided the basis for further exploration of the regulatory mechanism of GPx3 gene on reproductive traits of Qianbei ma goat.