油菜苗期耐盐碱性综合评价与根际土壤有机酸含量比较

为筛选耐盐碱油菜种质,研究有机酸在油菜抗盐碱过程中的生理作用,利用碱性盐（摩尔比为2:1的NaHCO₃和Na₂CO₃的混合物）进行处理,用模糊数学隶属函数分析法对8个油菜种质进行耐盐碱性综合评价,探究碱性盐耐性最强和最弱的油菜种质的根际土壤有机酸含量。结果表明,耐盐碱性综合评价为华油杂62>华双5号>崇2>16-P22外>青杂9号>金油158>17崇1>16-P32外。在碱性盐胁迫下耐性最强种质华油杂62和最弱种质16-P32外的根际土壤中检测到草酸、酒石酸、甲酸、苹果酸、抗坏血酸、乙酸、柠檬酸及琥珀酸8种有机酸。在碱性盐胁迫下,油菜根际土壤中草酸的积累量最高,抗坏血酸的积累量很低。华油杂62的各处理根际土壤有机酸含量总体高于16-P32外。

Comprehensive Evaluation of Saline-Alkali Tolerance and Comparison of Rhizosphere Soil Organic Acid Content at Rapeseed Seedling Stage

In order to screen the saline-alkali tolerant rapeseed germplasms and explore the physiological role of organic acids in saline-alkali resistance of rapeseed. The saline-alkali resistance of eight rapeseed germplasms treated with alkaline salt (a mixture of NaHCO₃ and Na₂CO₃ with a molar ratio of 2:1) were evaluated by fuzzy mathematical subordinative function analysis method, and then the organic acid content in rhizosphere soil of the two rapeseed germplasms with the strongest and weakest resistance to alkaline salt were investigated. The results showed that the strongest tolerant rapeseed germplasm was Huayouza 62 and the weakest was 16-P32 wai. The comprehensive evaluation of the tolerance was Huayouza 62 > Huashuang 5 > Chong 2 > 16-P22 wai > Qingza 9 > Jinyou 158 > 17 chong 1 > 16-P32 wai. Under alkaline salt stress condition, oxalic acid, tartaric acid, formic acid, malic acid, ascorbic acid, acetic acid, citric acid and succinic acid were detected in rhizosphere soil of Huayouza 62 and 16-P32 wai. Oxalic acid content was the highest, while the accumulation of ascorbic acid was very low. The contents of organic acids in rhizosphere soil of Huayouza 62 was generally higher than that of 16-P32 wai.