花生谷氨酸脱氢酶基因AhGDH1的克隆与生物信息学分析

运用电子克隆技术获得花生1个谷氨酸脱氢酶基因cDNA序列,同时设计引物以花生cDNA为模板进行扩增,经测序得到证实,命名为AhGDH1,GenBank登录号:KT933119。采用生物信息学方法,对该基因编码蛋白从氨基酸组成、基本理化性质、亚细胞定位、跨膜结构域、信号肽导肽、二级结构和三级结构等方面进行了预测和分析。结果表明:该基因cDNA序列长度为1 713bp,包含1个1 236bp开放阅读框,编码411个氨基酸;该编码蛋白含有谷氨酸脱氢酶两个典型的保守结构域。同源比对分析显示,该基因编码的氨基酸序列与鹰嘴豆等植物的谷氨酸脱氢酶基因具有高度的相似性,进一步确定该蛋白为谷氨酸脱氢酶。

Cloning and Bioinformatic Analysis of AhGDH1 Gene from Arachis hy pogaea L

A glutamate dehydrogenase gene from A rachis hypogaea L .was obtained by using the in silico cloning technique .Its sequence was amplified using primers designed according to the cDNA sequence .The sequencing confirmed and named the gene AhGDH1 with a GenBank accession number of KT933119. Through the bioinformatic analysis ,the composition of amino acid sequence ,basic physical and chemical properties ,subcellular localization , transmembrane region , signal peptide , guide peptide , and secondary and tertiary structures of the protein were studied .The results showed that the length of cDNA of the gene was 1 713 bp ,which included a 1 236 bp open reading frame and encoded 411 amino acids residue .The encoding protein of the AhGDH1 gene consisted of two typical conserved domains .The homology comparison and phylogenetic analysis indicated that the amino acid encoded by AhGDH1 gene in A .hypogaeawas highly homologous with those encoded by GDH1 gene in Cicer arietinum and others . Thus , it was determined that the protein was a glutamate dehydrogenase . The information provided for the expression analysis and function identification of GDH1 gene in A .hypogaea in the future .