鸭坦布苏病毒LH株E蛋白基因的克隆与结构

采用分子克隆技术对鸭坦布苏病毒(DTMUV)E蛋白基因进行克隆,同时运用多种生物学软件对该基因进行结构和生物学功能预测和分析.结果表明,本试验成功克隆了DTMUV LH株E蛋白基因,该基因包含一个1 500 bp的开放阅读框,编码500个氨基酸.遗传进化树分析表明,该蛋白与我国其他省份分离株的同源性很高.该蛋白的分子质量为54.3 ku,等电点为7.63,不存在信号肽序列,含有17个糖基化位点,有13个丝氨酸、4个络氨酸和8个苏氨酸的磷酸化位点.该蛋白存在跨膜区域,为亲水性蛋白.抗原位点分析表明,该蛋白有21个抗原肽段.本试验结果为E蛋白的生物学功能和致病机理的研究奠定重要的分子理论基础.

Gene clone and structure analysis of duck Tembusu virus strain LH E protein

Being the envelope protein of duck Tembusu virus ( DTMUV) , E protein plays a pivot role in viral assembly process and virus infection. To investigate the pathogenicity and management practice of DTMUV, structure and function of E protein were ana-lyzed. Results showed that E protein consisted of 1 500bp nucleotides and encoded 500 amino acids. Referring to phylogenetic analy-sis, E protein of strain LH was highly homologous with isolates from other provinces. Molecular weight of E protein gene was 54.3 ku, with 17 glycosylation sites, 13 Ser sites, 4 Tyr phosphorylation sites and 8 Threonine sites but without any signal peptide. In ad-dition, E protein transmembrane domain, a hydrophobic protein, consisted of 21 antigen sites. Secondary structure analysis of E pro-tein showed that the cyclic structure of beta turn of E protein was up to 47.80%.