樱桃番茄‘秋香’组织培养快繁技术研究

以温室种植的‘秋香’番茄健壮幼嫩侧芽为材料,研究4种不同消毒方法对番茄外植体的消毒效果,并通过继代培养,进一步研究并筛选‘秋香’番茄组织培养最佳培养基。试验结果表明:外植体消毒效果较理想的方式是10%的NaClO溶液(v/v)消毒20min,后用0.1%HgCl_2溶液(w/v)+2滴吐温20消毒3min;无菌株系建立适合的培养基为MS+BA 0.1mg·L~(-1)+IBA 0.1mg·L~(-1);继代培养适宜培养基配方为MS+BA0.1mg·L~(-1)+IBA 0.5mg·L~(-1),可以较快获得健壮的‘秋香’番茄试管苗。

Tissue Culture for Accelerated Propagation of Qiuxiang Cherry Tomato,Lycopersicon esculentum var.cerasiforme

Four methods for disinfecting the tomato explants were first compared on their efficacies prior toapplying the sanitized tender lateral buds from Qiuxiang tomatoes (Lycopersicon esculentum var.cerasiforme ) in the greenhouse for the propagation experiment.The selected disinfection treated the explants in a 10% NaClO solutionfor 20 min,followed by another of 0.1% mercuric chloride with a small amount of Tween 20 for 3 min. Theoptimized culture mediumfor establishing the aseptic lines consisted of MS+BA 0.1 mg·L-1 +IBA 0.1 ·L-1 . Whereas,the medium made of MS+BA 0.1 ·L-1 +IBA 0.5 ·L-1 was found to be suitable for the subculture toyield newhealthy and strong Qiuxiang tomato plantlets.