| 芸薹属种子贮藏蛋白质组分的比较研究 |
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| 引用本文： | 孔芳,蒋金金,吴磊,葛才林,王幼平. 芸薹属种子贮藏蛋白质组分的比较研究[J]. 中国油料作物学报, 2010, 32(1): 20-24 |
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| 作者姓名： | 孔芳 蒋金金 吴磊 葛才林 王幼平 |
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| 作者单位： | 扬州大学生物科学与技术学院，江苏 扬州225009
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| 摘 要： | 对组成芸薹属3个基本种和3个复合种的种子贮藏蛋白亚基组成进行了SDS-PAGE比较分析,结果表明:不同种间的种子贮藏蛋白具有一定的差异,表现出丰富的多态性;6个种共有24条亚基带,并各自具有特征蛋白谱带。利用贮藏蛋白亚基条带的信息,分析了种间的蛋白相似度,并进行了聚类分析。供试材料分为两类:Ⅰ类为白菜型油菜、甘蓝与甘蓝型油菜;Ⅱ类为黑芥、芥菜型油菜和埃塞俄比亚芥。
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| 关 键 词： | 芸薹属 种子贮藏蛋白 SDS-PAGE 蛋白质亚基 亲缘关系 |
Comparative study on seed storage protein of Brassica species |
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| KONG Fang,JIANG Jin-jin,WU Lei,GE Cai-lin,WANG You-ping. Comparative study on seed storage protein of Brassica species[J]. Chinese Journal of Oil Crop Sciences, 2010, 32(1): 20-24 |
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| Authors: | KONG Fang JIANG Jin-jin WU Lei GE Cai-lin WANG You-ping |
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| Affiliation: | KONG Fang,JIANG Jin-jin,WU Lei,GE Cai-lin,WANG You-ping(College of Bioscience , Biotechnology,Yangzhou University,Yangzhou 225009,China) |
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| Abstract: | Seed storage proteins extracted from six species of Brassica were analysed with SDS-PAGE.Seed storage proteins contained 24 subunits,which exhibited abundant polymorphisms and specific protein bands among different species. Cluster analysis indicated that 6 species were classified into two types. First type included B.rapa,B.oleracea and B.napus.The second type included B.nigra,B.juncea and B.carinata. |
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| Keywords: | SDS-PAGE Brassica Seed storage protein SDS-PAGE Protein subunits Genetic relationship |
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