不同虫生曲霉原生质体融合选育松墨天牛高毒力菌株

采用紫外或加热方法分别对虫生曲霉天牛生葡萄曲霉（Aspergillus vitis）和叶甲生烟曲霉（Aspergillus fumigatus var fumigatus）的原生质体分别进行灭活,然后对灭活双亲用PEG6000作融合剂进行原生质体融合。将融合子第2、第4、第6和第8代的菌落和分生孢子梗特征进行比较后,选出5株生长速度快、生产孢子多的融合株进行胞外蛋白酶的检测和松墨天牛幼虫毒力测试。结果发现,融合株U4-2对松墨天牛幼虫的死亡百分率为73.3%,和2个亲本相比较显著增强了毒力。

Screening for Aspergillus Strain with High Virulence to Monochamus alternatus by Protoplast Fusion

The protoplasts of Aspergillus vitis on Monochamus alternatus and Aspergillus fumigatus var fumigatus on Argopistes tsekooni were inactivated by ultraviolet or heating method, respectively. Then protoplast fusion with PEG6000 as fluxing agent was conducted on them. The fusions were compared by colony and conidial characters, growth rate and sporulation of the 2nd、4th、6th and 8th generations. Five fusions with fast growth and more sporulation were selected for extracellular protease detection on gelatin agar plate. The results of Monochamus alternatus larvae toxicity test showed that the lethal rate of fusion U4-2 strains to Monochamus alternatus larvae was 73.3%. The virulence was significantly enhanced compared with the 2 parent strains.