小麦品种对增强UV-B辐射响应的RAPD分析

在前一研究结果的基础上,选用8个对UV-B辐射耐受性不同的小麦品种(4个耐性品种和4个敏感品种),进一步对其进行RAPD分析。结果表明,8个小麦品种间存在着明显的遗传多态性。聚类分析显示,在遗传距离为0.35的水平上,可将它们明显区分出耐性和敏感两大类,这与其生长响应指数(RI)的判定结果基本一致。4个耐性品种共同具有1500bp(OPA-12)的特征谱带,这一分子标记可能与小麦耐UV-B辐射相关,有待进一步研究。

RAPD Analysis of Wheat Cultivars Responding to Enhanced UV-B Radiation

The decline in stratospheric ozone concentration has been confirmed by satellite measurements. Mathematical models predict a further increase in solar UV-B irradiation in further years. Effects of UV-B on plants have been the subjects of considerable research. An examination of more than 200 plant species reveals that roughly 20% are sensitive, 50% are mildly sensitive or tolerant and 30% are completely insensitive to UV-B radiation. In the past time, we reported that there were intraspecific differences in growth and grain yield response of 10 wheat cultivars to UV-B radiation, according to response index (RI), 4 sensitive cultivars and 4 tolerant cultivars have been determined. Plant species and even genotypes within species can differ greatly in their responses to UV-B. Based on the preceding research results, 8 wheat cultivars that responded differently to enhanced UV-B radiation, including 4 tolerant cultivars and 4 sensitive cultivars, were further made RAPD analysis. The results showed that there was obvious genetic polymorphism among 8 wheat cultivars. They were divided into two types at the level of 0.35 of genetic distance, that is, 4 cultivars were tolerant and other 4 were sensitive to enhanced UV-B radiation. This classification was consistent with the result of RI. As a common DNA band of 4 tolerant cultivars, 1 500bp (OPA-12) was correlated with UV-B-resistibility of wheat, which needed further study.