Novel interspecific hybridization between sweetpotato (<Emphasis Type="Italic">Ipomoea batatas</Emphasis> (L.) Lam.) and its two diploid wild relatives |
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Authors: | Qinghe Cao An Zhang Daifu Ma Hongmin Li Qiang Li Peng Li |
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Institution: | (1) Institute of Sweetpotato Research, Chinese Academy of Agricultural Sciences, 221121 Xuzhou, China;(2) Jiangsu Xuzhou Sweetpotato Research Centre, 221121 Xuzhou, China;(3) Xuzhou Academy of Agricultural Sciences, 221121 Xuzhou, China |
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Abstract: | Interspecific hybridization is an important approach to broaden the genetic base and create novel plant forms in breeding
programs. However, interspecific hybridization in Ipomoea is very difficult due to the cross incompatibility. Here we report two novel interspecific F1 hybrids between I. batatas (L.) Lam. (2n = 6x = 90) and two wild species, I. grandifolia (2n = 2x = 30) and I. purpurea (2n = 2x = 30). Hybridization was stimulated by applying plant growth hormones. Morphological, molecular and cytological tests were
conducted to confirm their hybridity. We found that the two hybrids were quite distinctive in leaf color and morphology, and
yielded intermediate sizes of storage roots compared to their respective parents. Inter-simple sequence repeat analysis showed
that the unique DNA bands from the wild parents could be detected in these two hybrids. The cluster analysis also showed that
the two F1 hybrids were closer to I. batatas in phylogeny relationship. The number of chromosomes of the two hybrids was both 60, indicating that the hybrids were tetraploid.
The meiotic configuration analysis of the H1 of I. batatas × I. grandifolia revealed the occurrence of 17.58I + 14.28II + 1.36III + 2.48IV at metaphase I in average chromosome association per pollen
mother cells (PMCs), 4.26I + 18.32II + 2.56III + 3.12IV was average meiotic configuration in the H2 of I. batatas × I. purpurea. Both hybrids appeared to be polyads and multi-microcytes at tetrad phase and differed in their pollen fertility. |
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Keywords: | Interspecific hybrids Morphology Molecular Cytology |
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