| 基于CE-AFLP的5个美洲黑杨新品种指纹图谱分析 |
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| 引用本文： | 贾会霞,胡建军,卢孟柱.基于CE-AFLP的5个美洲黑杨新品种指纹图谱分析[J].林业科学研究,2013,26(3):281-286. |
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| 作者姓名： | 贾会霞 胡建军 卢孟柱 |
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| 作者单位： | 林木遗传育种国家重点实验室,中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091 |
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| 基金项目： | 林业行业公益类重大专项(201304103);"863"计划(2011AA100201);林木遗传育种国家重点实验室专项课题(CAFYBB2012041) |
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| 摘 要： | 以5个美洲黑杨新品种(丹红杨、南杨、中林2025杨、中红杨和全红杨)为实验材料,利用CE-AFLP(毛细管电泳-AFLP)技术,采用EcoR I+3/Mse I+3和Pst I+2/Mse I+3引物组合构建指纹图谱,并进行遗传多样性分析.结果表明:从36对引物组合中筛选出12对扩增条带数目多、稳定且清晰的引物进行扩增,共获得条带数877条,多态性条带数315条,平均多态率为35.92％.采用UPGMA法进行聚类分析,相似系数为0.74～1.00;丹红杨与南杨之间的差异较大;在相似系数0.98时,中林2025杨与中红杨和全红杨分为两类,11条稳定特异的条带可以作为鉴别它们的依据;全红杨和中红杨之间未监测到稳定清晰的特异条带,初步认为2个品种在基因组水平上没有明显差异.
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| 关 键 词： | CE-AFLP 美洲黑杨 遗传多样性 指纹图谱 |
| 收稿时间： | 2012/1/16 0:00:00 |
Fingerprint Analysis of Five New Cultivars of Populus deltoides Based on CE-AFLP |
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| JIA Hui-xi,HU Jian-jun and LU Meng-zhu.Fingerprint Analysis of Five New Cultivars of Populus deltoides Based on CE-AFLP[J].Forest Research,2013,26(3):281-286. |
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| Authors: | JIA Hui-xi HU Jian-jun and LU Meng-zhu |
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| Institution: | State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry,
Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Beijing 100091, China;State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry,
Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Beijing 100091, China;State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry,
Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Beijing 100091, China |
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| Abstract: | |
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| Keywords: | CE-AFLP Populus deltoides fingerprint genetic diversity |
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