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| 抗Asia1型口蹄疫病毒单克隆抗体的制备及生物学特性鉴定 | |
| 引用本文： | 李万,周国辉,杨增岐,涂亚斌,于力. 抗Asia1型口蹄疫病毒单克隆抗体的制备及生物学特性鉴定[J]. 中国预防兽医学报, 2007, 29(6): 448-452 |
| 作者姓名： | 李万 周国辉 杨增岐 涂亚斌 于力 |
| 作者单位： | 1. 西北农林科技大学,动物科技学院,陕西,杨凌,712100;中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/大动物病研究室,黑龙江,哈尔滨,150001 2. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/大动物病研究室,黑龙江,哈尔滨,150001 3. 西北农林科技大学,动物科技学院,陕西,杨凌,712100 |
| 摘 要： | 用纯化的Asia1型口蹄疫病毒免疫BALB/c小鼠,取免疫小鼠脾细胞与SP2/0骨髓瘤细胞进行融合,经间接ELISA和间接免疫荧光(IFA)筛选,有限稀释法克隆,获得了2株稳定分泌单克隆抗体的杂交瘤细胞株,分别命名为3H6、5G3,其细胞培养上清效价分别为1:64和1:128,小鼠腹水效价分别为1×10~(-4)和8×10~(-3);ELISA和IFA结果显示,2株单抗仅与Asial型口蹄疫病毒反应,不与O型口蹄疫病毒反应,表明它们均为抗Asial型口蹄疫病毒的型特异性单克隆抗体。westem blot结果显示,2株单克隆抗体均不与全病毒抗原反应,表明它们所针对的抗原表位均为构象表位。相加ELISA试验表明,两株单抗识别不同的抗原表位。经硫氰酸盐洗脱法测定,3H6和5G3的相对亲和力指数分别为1.0 mol/L和1.5 mol/L。这2株单抗的获得为建立口蹄疫病毒检测方法提供了强有力的工具。 |
| 关 键 词： | 口蹄疫病毒 单克隆抗体 |
| 文章编号： | 1008-0589（2007）06-0448-05 |
| 修稿时间： | 2007-01-12 |
Production and characterization of monoclonal antibodies against Asia1 foot-and-mouth disease virus |
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| LI Wan,ZHOU Guo-hui,YANG Zeng-qi,TU Ya-bin,YU Li. Production and characterization of monoclonal antibodies against Asia1 foot-and-mouth disease virus[J]. Chinese Journal of Preventive Veterinary Medicine, 2007, 29(6): 448-452 | |
| Authors: | LI Wan ZHOU Guo-hui YANG Zeng-qi TU Ya-bin YU Li |
| Affiliation: | 1. College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China; 2. Division of Livestock Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China |
| Abstract: | Monoclonal antibodies(McAbs)3H6 and 5G3 against FMDV(Foot-and mouth disease virus)serotype Asial were produced by fusing SP2/0 myeloma cells with spleen cells of BALB/c mice immunized with purified viruses.Both McAbs reacted with FMDV serotype Asial but not with FMDV serotype O,as determined by Enzyme-linked immunsorbent assay(ELISA) and indirect immunofluorescence assay(IFA).Western blot analysis showed that both McAbs recognized conformational epitopes but not whole viral proteins.The relative affinity index(RAI)of the McAbs were 1.0 mol/L and 1.5 mol/L,respectively,as indicated by thiocyanate elution measurement.The result of additive ELISA showed that the McAbs could recognize different antigen epitopes.The McAbs are useful tools for developing diagnostic techniques of foot-and-mouth disease virus serotype Asial. |
| Keywords: | foot-and-mouth disease virus monoclonal antibodies |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |