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| 15N同位素稀释法研究固氮菌接种对甘蔗生物固氮的影响 | |
| 引用本文： | 罗霆,欧阳雪庆,杨丽涛,李杨瑞.15N同位素稀释法研究固氮菌接种对甘蔗生物固氮的影响[J].核农学报,2010,24(5):1026-1031. |
| 作者姓名： | 罗霆 欧阳雪庆 杨丽涛 李杨瑞 |
| 作者单位： | 1. 广西大学广西亚热带生物资源保护利用重点实验室,广西,南宁,530004;广西农业科学院能源作物研究所,广西,南宁,530007;广西作物遗传改良生物技术重点实验室,广西,南宁,530007;中国农业科学院甘蔗研究中心,广西,南宁,530007 2. 广西大学广西亚热带生物资源保护利用重点实验室,广西,南宁,530004 3. 广西作物遗传改良生物技术重点实验室,广西,南宁,530007;中国农业科学院甘蔗研究中心,广西,南宁,530007 |
| 基金项目： | 国家自然科学基金项目（30660085），国家科技支撑计划项目（2007BAD30B03），广西科技攻关重点项目（桂科攻0782004-3），广西科技攻关项目（桂科能0815011） |
| 摘 要： | 利用15N同位素稀释法，研究接种固氮菌klebsiella L03对甘蔗品种B8和ROC22的生物固氮的影响。结果表明： B8的固氮百分率最高为3128%Ndfa，从苗期开始就显著高于ROC22，分蘖期和伸长初期的固氮量和固氮百分率与ROC22相比差异达到极显著水平。B8和ROC22的根、茎、叶都可发生固氮，以伸长初期（60d）叶片中的固氮能力最强。接种L03能显著提高B8和ROC22各器官的含氮量，其中对ROC22叶片和B8茎的含氮量提高作用最明显。不同甘蔗品种、相同品种的不同器官甚至相同器官的不同生长时期固氮作用有很大差异。 |
| 关 键 词： | 甘蔗 生物固氮 15N同位素稀释法 固氮百分率 含氮量 |
EFFECT OF NITROGEN-FIXING BACTERIA INOCULATION ON BIOLOGICAL NITROGEN FIXATION IN SUGARCANE BY 15N ISOTOPE DILUTION TECHNIQUE |
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| LUO Ting,OUYANG Xue-qing,YANG Li-tao,LI Yang-rui.EFFECT OF NITROGEN-FIXING BACTERIA INOCULATION ON BIOLOGICAL NITROGEN FIXATION IN SUGARCANE BY 15N ISOTOPE DILUTION TECHNIQUE[J].Acta Agriculturae Nucleatae Sinica,2010,24(5):1026-1031. | |
| Authors: | LUO Ting OUYANG Xue-qing YANG Li-tao LI Yang-rui |
| Institution: | 1Guangxi Key Laboratory for Conservation and Utilization of Subtropical Bioresources, Guangxi University, Nanning, Guangxi 530004; 2Energy Crop Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007; 3Guangxi Crop Genetic Improvement and Biotechnology Lab., Nanning, Guangxi 530007; 4Sugarcane Research Institute, Chinese Academy of Agricultural Sciences, Nanning, Guangxi 530007 |
| Abstract: | 5N stable\|isotope dilution technique was used to study effect of nitrogen-fixing bacteria strains inoculation on biological nitrogen fixation (BNF) in sugarcane varieties B8 and ROC22. The results showed that there were significant differences of %Ndfa (Nitrogen-fixing percentage) at different development stages between B8 and ROC22. The %Ndfa of B8 was obviously higher than that of ROC22 at young shoot stage, tillering stage and early elongation stage, and the highest %Ndfa of B8 reached 3128%Ndfa. BNF was found in root, stem and leaf but the maximum BNF was in leaf at 60d. Inoculation with L03 could increase total nitrogen content in both B8 and ROC22. The leaf of ROC22 and the stem of B8 were found benefited most from the inoculation. BNF varied significantly in different varieties, organs and growth stages of sugarcane. |
| Keywords: | sugarcane biological nitrogen fixation 15 N isotope dilution technique nitrogen-fixing percentage total nitrogen content |
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