Comparative detection of mild strains of potato spindle tuber viroid from the dormant potato tubers by Return-polyacrylamiee gel electrophoresis and nucleic acid hybridization |
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Authors: | R. P. Singh A. Boucher |
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Affiliation: | (1) Agriculture Canada, Research Branch, Research Station, P.O. Box 20280, E3B 4Z7 Fredericton, New Brunswick, Canada |
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Abstract: | Summary Using a modified procedure of Return-polyacrylamide gel electrophoresis (R-PAGE) mild strains of potato spindle tuber viroid (M-PSTV) were detected reliably from dormant tubers. The sensitivity of R-PAGE detection of M-PSTV was equivalent to that of nucleic acid hybridization. Both methods detected M-PSTV when infected tissue was mixed with healthy tissue in a ratio of 1 to 100. When extracted nucleic acid was diluted with buffer, R-PAGE detected PSTV at a dilution of 1:256 and nucleic hybridization only up to 1:64 PSTV was readily detected from 18 potato cultivars. In addition, mild, ‘intermediate’ and severe strains were separated by R-PAGE, on the basis of their mobility on the electrophorogram. |
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Keywords: | sensitivity routine testing silver staining dot-blot severe strain separation of strains |
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