Comparative detection of mild strains of potato spindle tuber viroid from the dormant potato tubers by Return-polyacrylamiee gel electrophoresis and nucleic acid hybridization |
| |
Authors: | R P Singh A Boucher |
| |
Institution: | (1) Agriculture Canada, Research Branch, Research Station, P.O. Box 20280, E3B 4Z7 Fredericton, New Brunswick, Canada |
| |
Abstract: | Summary Using a modified procedure of Return-polyacrylamide gel electrophoresis (R-PAGE) mild strains of potato spindle tuber viroid
(M-PSTV) were detected reliably from dormant tubers. The sensitivity of R-PAGE detection of M-PSTV was equivalent to that
of nucleic acid hybridization. Both methods detected M-PSTV when infected tissue was mixed with healthy tissue in a ratio
of 1 to 100. When extracted nucleic acid was diluted with buffer, R-PAGE detected PSTV at a dilution of 1:256 and nucleic
hybridization only up to 1:64 PSTV was readily detected from 18 potato cultivars. In addition, mild, ‘intermediate’ and severe
strains were separated by R-PAGE, on the basis of their mobility on the electrophorogram. |
| |
Keywords: | sensitivity routine testing silver staining dot-blot severe strain separation of strains |
本文献已被 SpringerLink 等数据库收录! |
|