Improved diagnosis of powdery scab (Spongospora subterranea f.sp.subterranea) symptoms on potato tubers (Solanum tuberosum L.) |
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Authors: | Eisse G De Haan Gé w Van Den Bovenkamp |
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Institution: | (1) Dutch General Inspection Service for Agricultural seed and seed potatoes (NAK), Randweg 14, 8300 BC Emmeloord, The Netherlands |
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Abstract: | Summary Based on visual inspection, discrimination between common scab (Streptomyces spp.) and powdery scab (Spongospora subterranea) can be difficult. Inspections are performed on unwashed samples, incidentally supported by microscopic examination.
During 1994–1996 surveys were performed in The Netherlands on tubers with symptoms resembling common scab. Under microscopic
assessment nearly all samples showed the presence of structures resembling cystosori (sporeballs) ofS. subterranea. At that time confirmation using alternative techniques was not possible.
In 2003 research was undertaken to clarify the situation with respect to scab on potato tubers in The Netherlands. One hundred
and eighteen scab samples were extensively tested forS. subterranea. All samples were digitally photographed, microscopically examinated and tested with real-time PCR and DAS-ELISA. Use of
these modern methods resulted in a clear picture of symptoms that can be attributed toS. subterranea. A lot of scab samples with structures resembling cystosori could not be confirmed as contaminated withS. subterranea. |
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Keywords: | common scab cystosori ELISA inspections real-time PCR tuber symptoms |
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