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铅胁迫对黄瓜幼苗叶片蛋白质的影响
引用本文:刘素纯,萧浪涛,廖柏寒,Annie Zhujiang,Connie Lo. 铅胁迫对黄瓜幼苗叶片蛋白质的影响[J]. 湖南农业大学学报(自然科学版), 2006, 32(2): 141-144
作者姓名:刘素纯  萧浪涛  廖柏寒  Annie Zhujiang  Connie Lo
作者单位:湖南农业大学湖南省植物激素与生长发育重点实验室,湖南,长沙,410128;湖南农业大学资源环境学院,湖南,长沙,410128;加州学洛杉矶分校化学系,加利福尼亚州,洛杉矶,90024;加州学洛杉矶分校神经科学系,加利福尼亚州,洛杉矶,90024
基金项目:教育部青年骨干教师基金(2000科教司65号),湖南省教育厅青年基金(99B05)
摘    要:黄瓜幼苗经不同质量浓度铅处理后,叶片中可溶性蛋白质含量均发生了明显的变化.100 mg/L铅处理时,黄瓜幼苗叶片中可溶性蛋白质含量高于对照值,且随处理时间的延长,与对照比增加的值逐渐减少;500 mg/L和900 mg/L铅处理的叶片中可溶性蛋白质含量随着处理时间的延长表现为下降趋势,并且铅质量浓度越高,随着处理时间的延长,蛋白质含量下降量增加.不同质量浓度铅处理不同时期黄瓜幼苗叶片可溶性蛋白质组分的变化在谱带数和表达量上有明显的差异.

关 键 词:黄瓜  铅胁迫  蛋白质  SDS-PAGE电泳
文章编号:1007-1032(2006)02-0141-04
修稿时间:2005-11-24

Effects of Lead Stress on Proteins in Cucumber Seedling Leaves
LIU Su-chun,XIAO Lang-tao,LIAO Bo-han,Annie Zhujiang,Connie Lo. Effects of Lead Stress on Proteins in Cucumber Seedling Leaves[J]. Journal of Hunan Agricultural University, 2006, 32(2): 141-144
Authors:LIU Su-chun  XIAO Lang-tao  LIAO Bo-han  Annie Zhujiang  Connie Lo
Abstract:The content change of soluble protein in leaves was obviously token place after lead treatment of different mass concentration in cucumber seedling.The content of soluble protein in leaves was higher to control at lead stress of 100 mg/Lt,he increase amount compared to control showed decrease with the increase of treatment time.The content of soluble protein in leaves showed a downtrend with the increase of treatment times at lead stress of 500 mg/L and 900 mg/L,the decreased content of protein was increased with the increase lead concentration and treatment time.The change of proteomics is evidently different on isoenzymic band numbers and expression at different mass concentration and different period under lead stress.It implies that the synthesis and degradation of protein are related to the anti-lead toxicity in cucumber seedlings.
Keywords:cucumber  lead stress  protein  SDS-PAGE electrophoresis
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