| 反相高效液相色谱法测定不同品质栀子中栀子苷的含量 |
| |
| 引用本文： | 饶黄云,邹盛勤.反相高效液相色谱法测定不同品质栀子中栀子苷的含量[J].安徽农业科学,2006,34(17):4207-4208. |
| |
| 作者姓名： | 饶黄云 邹盛勤 |
| |
| 作者单位： | 东华理工学院物理系,江西,抚州,344000;江西省天然药物活性成分研究重点实验室,宜春学院生物工程研究所,江西,宜春,336000 |
| |
| 摘 要： | 建立反相高效液相色谱-光电二极管阵列检测器法测定了不同品质栀子生品中栀子苷的含量。采用Nucleodur C18色谱柱(4.6mm×250 mm,5μm),以甲醇-水(22∶78,体积比)为流动相,流速1.0 ml/min,检测波长240 nm,柱温28℃。栀子苷在0.80~7.20μg范围内呈良好的线性关系(r=0.999 98),加样回收率为99.3%。该方法简便、快速、准确,可用于栀子生品的质量控制。
|
| 关 键 词： | 栀子 栀子苷 反相高效液相色谱(HPLC) 二极管阵列检测器 峰纯度 |
| 文章编号： | 0517-6611（2006）17-4207-02 |
| 收稿时间： | 06 2 2006 12:00AM |
| 修稿时间： | 2006-06-02 |
Determination of Geniposide in Fruclus Gardeniae of Different Quality with HPLC |
| |
| RAO Huang-yun et al.Determination of Geniposide in Fruclus Gardeniae of Different Quality with HPLC[J].Journal of Anhui Agricultural Sciences,2006,34(17):4207-4208. |
| |
| Authors: | RAO Huang-yun |
| |
| Abstract: | HPLC method coupled with photodiode array detection(PAD) for the determination of geniposide in Fructus Gardeniae of different quality was established.The separation was achieved on a Nucleodur C_(18) column(4.6 mm×250 mm,5 μm)at 28?℃ through the application of the methanol-water(22∶78)as the mobile phase at a flow rate of 1.0 ml/min and 240 nm as the detection wavelength.Geniposide showed good relationship at the range of 0.80～7.20 μg(r=0.999?98).The recovery was 99.3 %.The proposed method was simple,rapid and accurate and promised to be applicable for the quality control of Fructus Gardeniae. |
| |
| Keywords: | Fructus Gardeniae Geniposide RP-HPLC Photodiode array detection Peak purity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
