PPD的批次与注射方式对PPD皮内变态反应检疫的影响

为了探究在牛结核病检疫中出现的使用不同批次的PPD和皮下注射PPD等情况是否会对检疫结果的判定造成干扰。本试验首次通过在豚鼠背部采用皮下注射法和皮内交替注射同一厂家不同批次的结核菌素提纯蛋白衍化物(Purified protein derivative of tuberculin,PPD)的方法,参照《结核菌素提纯蛋白衍化物(PPD)制造及检定规程》于每次注射后24h、48h和72h观察皮试处是否产生迟发型变态反应。试验结果为皮内组(不同批次PPD皮内注射)和皮下组(同一批次PPD皮下注射)经3次注射后的皮试结果与标准参照组的皮试结果无差异,且在每次皮试后的24h、48h和72h皮试处均未出现局部红斑硬结。本试验证明了PPD皮内变态反应检疫结果判定不受PPD皮下接种及PPD的不同批次的干扰。

The effect of PPD batch and injection method on Tuberculin intradermal allergy test

In order to explore whether the use of different batches of PPD and subcutaneous injection of PPD in bovine tuberculosis quarantine will interfere with the determination of quarantine results. For the first time, the method of subcutaneous injection and intracutaneous injection of different batches of standard tuberculin(Purified protein derivative of tuberculin,PPD)from the same manufacturer were used on the back of guinea pigs. The delayed type allergic reaction was observed 24 h, 48 h and 72 h after each skin test according to the regulation for manufacture and verification of purified protein derivative of tuberculin (PPD).The results showed that there was no difference between the skin test results of the intradermal group (intradermal injection of different batches of PPD) and the subcutaneous group (subcutaneous injection of the same batch of PPD) after three injections and the skin test results of the standard reference group, and there was no local erythema at 24h, 48H and 72h after each skin test.This test proved that the quarantine result of PPD intradermal allergy was not interfered by PPD subcutaneous inoculation and different batches of PPD.