| 贵州栽培苦参中苦参碱的含量测定 |
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| 引用本文： | 敖茂宏,宋智琴,罗晓青,王代谷. 贵州栽培苦参中苦参碱的含量测定[J]. 广东农业科学, 2009, 0(11): 118-119 |
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| 作者姓名： | 敖茂宏 宋智琴 罗晓青 王代谷 |
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| 作者单位： | 贵州省亚热带作物研究所,贵州,兴义,562400,贵阳中医学院,贵州,贵阳,550002 |
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| 摘 要： | 为建立贵州栽培苦参药材中有效成分苦参碱含星的测定方法,采用HPLC法DiamonsiITM(钻石)C18色谱柱,以乙睛、3%磷酸(70:30)为流动相,流速为1.0 mL/min,柱温25℃,检测波长210 nm.结果显示方法线性关系良好,平均加样回收率98.01%,RSD=1.15%,表明该法简便快速,结果准确,可以作为控制栽培苦参药材质量的方法.
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| 关 键 词： | 苦参 苦参碱 |
Determination of Matrine in cultivated Sophora flavescens Ait in Guizhou province |
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| AO Mao-hong,SONG Zhi-qin,LUO Xiao-qing,WANG Dai-gu. Determination of Matrine in cultivated Sophora flavescens Ait in Guizhou province[J]. Guangdong Agricultural Sciences, 2009, 0(11): 118-119 |
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| Authors: | AO Mao-hong SONG Zhi-qin LUO Xiao-qing WANG Dai-gu |
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| Affiliation: | AO Mao-hong1,SONG Zhi-qin2,LUO Xiao-qing1,WANG Dai-gu1(1.Subtropical Crops Research Institute of Guizhou Province,Xingyi 562400,China,2.Guiyang College of Traditional Chinese Medicine,Guiyang 550002,China) |
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| Abstract: | To establish the determination method of Matrine in Sophora flavescens Ait.DiamonsiITMC18 column was used and Acetonitrile-3%Phosphate acetate(70:30)was used as a mobile phase,velocity-1.0 mL/min;column temperature-25℃,the detective wavelength was 210 nm.This method showed a good linear relationship.The average recoveries was 98.01%,RSD was 1.15%.This method is simple,rapid,accurate,and can be used as the quantitative analysis method for this preparation. |
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| Keywords: | HPLC HPLC Sophora flavescens Ait Matrine |
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