| 网纹甜瓜试管苗玻璃化成因研究 |
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| 引用本文： | 齐红岩,贾卓男,陈岩.网纹甜瓜试管苗玻璃化成因研究[J].河南农业科学,2009(3). |
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| 作者姓名： | 齐红岩 贾卓男 陈岩 |
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| 作者单位： | 沈阳农业大学,园艺学院,辽宁省设施园艺重点实验室,辽宁,沈阳,110161 |
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| 摘 要： | 以日本伯爵系列网纹甜瓜R97和国内网纹甜瓜品种顶峰3号为试材,探讨了组培过程中不同封口材料、继代次数、培养条件、琼脂用量、蔗糖浓度和激素水平对试管苗玻璃化的影响。结果表明,以塑料薄膜为封口材料、增加继代次数、高温和低光强、较低的蔗糖和琼脂用量、较高浓度的6-BA均会导致试管苗玻璃苗百分率的增加,并初步得出试管苗玻璃化的防治措施。
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| 关 键 词： | 网纹甜瓜 组织培养 玻璃化现象 防治措施 |
Causes of Vitrification of Netted Melon Plantlets(Cucumis melo L.) |
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| QI Hong-yan,JIA Zhuo-nan,CHEN Yan.Causes of Vitrification of Netted Melon Plantlets(Cucumis melo L.)[J].Journal of Henan Agricultural Sciences,2009(3). |
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| Authors: | QI Hong-yan JIA Zhuo-nan CHEN Yan |
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| Institution: | College of Horticulture;Shenyang Agricultural University;Key Laboratory of Protected Horticulture of Liaoning Province;Shenyang 110161;China |
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| Abstract: | The netted melon cultivar R97(belong to Japanese duke series)and Dingfeng No.3(belong to the domestic variety)were used for the tissue culture to study the effects of different seal material,different number of subculture,culture condition,different concentration of sucrose and agar and plant growth regulators concentration on vitro plantlet vitrification of melon.The results showed that using plastic membrane to seal,increasing number of subculture,high temperature,low light intensity,decreasing concentrat... |
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| Keywords: | Netted melon Tissue culture Vitrification phenomenon Prevent measures |
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