| 浊点萃取-高效液相色谱法测定虾肉中孔雀石绿和结晶紫残留的研究 |
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| 引用本文： | 陈建伟,姚志云,毛健伟,莫小荣,赵道远,杨明敏.浊点萃取-高效液相色谱法测定虾肉中孔雀石绿和结晶紫残留的研究[J].南京农业大学学报,2010,33(1). |
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| 作者姓名： | 陈建伟 姚志云 毛健伟 莫小荣 赵道远 杨明敏 |
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| 作者单位： | 南京农业大学理学院,江苏,南京,210095 |
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| 摘 要： | 利用浊点萃取方法富集孔雀石绿和结晶紫,建立了用高效液相色谱(二极管阵列检测器)同时测定虾肉中孔雀石绿和结晶紫残留的方法,并研究了表面活性剂、盐、平衡时间及平衡温度对浊点萃取效率的影响。该分析方法应用于虾中两种物质的残留分析,结果表明:在虾肉中分别添加3个不同浓度的孔雀石绿和结晶紫,添加回收率范围分别为75.5%～80.2%(孔雀石绿)和81.4%～89.6%(结晶紫),相对标准偏差(RSD)分别为2.3%～3.1%(孔雀石绿)和1.5%～3.3%(结晶紫)。该方法的检测限分别为孔雀石绿2.0μg·kg~(-1)、结晶紫1.0μg·kg~(-1),可满足我国和欧盟对虾中孔雀石绿和结晶紫的检测要求。
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| 关 键 词： | 浊点萃取 高效液相色谱 虾 孔雀石绿 结晶紫 |
Determination of residues of malachite green and crystal violet in shrimp samples by cloud point extraction-high performance liquid chromatography |
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| CHEN Jian-wei,YAO Zhi-yun,MAO Jian-wei,MO Xiao-rong,ZHAO Dao-yuan,YANG Ming-min.Determination of residues of malachite green and crystal violet in shrimp samples by cloud point extraction-high performance liquid chromatography[J].Journal of Nanjing Agricultural University,2010,33(1). |
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| Authors: | CHEN Jian-wei YAO Zhi-yun MAO Jian-wei MO Xiao-rong ZHAO Dao-yuan YANG Ming-min |
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| Institution: | College of Sciences/a>;Nanjing Agricultural University/a>;Nanjing 210095/a>;China |
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| Abstract: | A cloud point extraction(CPE) method was used to separate and concentrate malachite green(MG) and crystal violet (CV) in shrimp samples followed by HPLC-diode-array detector(DAD) analysis was developed.The effects of surfactant,salt additive,equilibration time and the equilibration temperature on extraction efficiency were studied.The method was applied to determine residues in shrimp samples.Under optimum conditions,recoveries of spiked shrimp sample,relative standard deviations (RSD) and the limit of dete... |
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