| 草鱼出血病病毒854株基因组SDS—PAGE分析及其核酸类型鉴定 |
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| 引用本文： | 曾令兵,左文功.草鱼出血病病毒854株基因组SDS—PAGE分析及其核酸类型鉴定[J].淡水渔业,1991(5):17-19. |
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| 作者姓名： | 曾令兵 左文功 |
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| 作者单位： | 中国水产科学研究院长江水产研究所，中国水产科学研究院长江水产研究所 |
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| 摘 要： | 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)结果表明:草鱼出血病病毒854株基因组由11个分段的核酸片段组成,总分子量约为14.46×10~6道尔顿。其中最大片段的分子量约为2.45×10~6道尔顿;最小片段的分子量约为0.38×10~6道尔顿。其基因组核酸片段电泳图谱具有病毒株的特异性.核酸酶敏感性试验表明该病毒的核酸为RNA。
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| 关 键 词： | 草鱼出血病病毒 核酸型 基因组 电泳图谱 |
Genome SDS—PAGE analysis and nucleic acid identification of grass carp hemorrhage virus strain 854 |
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| Zeng Lingbing Zuo Wengong.Genome SDS—PAGE analysis and nucleic acid identification of grass carp hemorrhage virus strain 854[J].Freshwater Fisheries,1991(5):17-19. |
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| Authors: | Zeng Lingbing Zuo Wengong |
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| Abstract: | SDS-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the genome of
grass carp hemorrhage virus strain 854 was composed of 11 segments of dsRNA The molecular weight
of the segments ranged from 2. 45×10~6 to 0. 38×10~6 daltons with a total genome of approximately
14. 46×10~6 daltons The electrophoresis pattern of the viral genome has strain-specific characteristics
The sensitivity test to nuclaase revealed that the viral nucleic acid is RNA. |
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| Keywords: | grass carp hemorrhage virus nucleic acid type genome |
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