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Characterisation of a SAT-1 outbreak of foot-and-mouth disease in captive African buffalo (Syncerus caffer): clinical symptoms, genetic characterisation and phylogenetic comparison of outbreak isolates |
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| Authors: | Vosloo W de Klerk L-M Boshoff C I Botha B Dwarka R M Keet D Haydon D T |
| Institution: | aOnderstepoort Veterinary Institute, Private Bag X05, Onderstepoort 0110, South Africa bDepartment of Veterinary Tropical Diseases, University of Pretoria, Pretoria 0002, South Africa cVeterinary Wildlife Services, Kruger National Park, P.O. Box 156, Skukuza 1350, South Africa dDepartment of Biomedical Sciences, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa eOffice of the State Veterinarian, Kruger National Park, PO Box 12, Skukuza 1350, South Africa fDivision of Environmental and Evolutionary Biology, University of Glasgow, Glasgow G12 8QQ, UK |
| Abstract: | African buffalo (Syncerus caffer) play an important role in the maintenance of the SAT types of foot-and-mouth disease (FMD) in southern Africa. These long-term carriers mostly become sub-clinically infected, maintaining the disease and posing a threat to other susceptible wildlife and domestic species. During an unrelated bovine tuberculosis experiment using captive buffalo in the Kruger National Park (KNP), an outbreak of SAT-1 occurred and was further investigated. The clinical signs were recorded and all animals demonstrated significant weight loss and lymphopenia that lasted 100 days. In addition, the mean cell volume and mean cell haemoglobin values were significantly higher than before the outbreak started. Virus was isolated from several buffalo over a period of 167 days post infection and the molecular clock estimated to be 3 × 10−5 nucleotide substitutions per site per day. Seven amino acid changes occurred of which four occurred in hypervariable regions previously described for SAT-1. The genetic relationship of the outbreak virus was compared to buffalo viruses previously obtained from the KNP but the phylogeny was largely unresolved, therefore the relationship of this outbreak strain to others isolated from the KNP remains unclear. |
| Keywords: | Foot-and-mouth disease African buffalo Clinical disease Phylogenetic analysis Rate of change |
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