| PAS-ELISA检测GFLV和GLRaV-3技术的研究 |
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| 引用本文： | 陈建军,曹香林,李学梅,刘崇怀,古勤生. PAS-ELISA检测GFLV和GLRaV-3技术的研究[J]. 河南农业科学, 2007, 0(2): 50-53 |
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| 作者姓名： | 陈建军 曹香林 李学梅 刘崇怀 古勤生 |
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| 作者单位： | 1. 河南师范大学,生命科学学院,河南,新乡,453007
2. 中国农业科学院,郑州果树研究所,河南,郑州,450009 |
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| 摘 要： | 以中国农业科学院郑州果树研究所国家葡萄资源圃的葡萄品种为试材,对葡萄扇叶病毒(GFLV)和葡萄卷叶病毒Ⅲ(GLRaV-3)进行了A蛋白酶联免疫吸附测定(PAS-ELISA)的检测技术的研究。从阴、阳性对照及抗血清或抗体浓度的选择,到不同时期、检测部位对结果的影响,建立了一套完整的ELISA检测GFLV和GLRaV-3程序;通过试验分析,该方法快速,简便,经济且灵敏度高,不失为葡萄病毒检测的一种新方法。通过检测,还了解到GFLV的发病高峰在春季,主要发病部位在上部叶片,而GLRaV-3的发病高峰在秋季,重要发病部位在下部叶片。
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| 关 键 词： | 葡萄 病毒检测 PAS-ELISA 灵敏度 GFLV GLRaV-3 |
| 文章编号： | 1004-3268(2007)02-0050-04 |
| 修稿时间： | 2006-09-16 |
Study on Detecting GFLV and GLRaV- 3 by PAS-ELISA |
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| CHEN Jian-jun,CAO Xiang-lin,LI Xue-mei,LIU Chong-huai,GU Qin-sheng. Study on Detecting GFLV and GLRaV- 3 by PAS-ELISA[J]. Journal of Henan Agricultural Sciences, 2007, 0(2): 50-53 |
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| Authors: | CHEN Jian-jun CAO Xiang-lin LI Xue-mei LIU Chong-huai GU Qin-sheng |
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| Affiliation: | 1. College of Life Science, Henan Normal University. Xinxiang 453007, China; 2. Zhengzhou Fruit Research Institute,CAAS,Zhengzhou 450009,China |
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| Abstract: | The techniques of detecting grapevine fanleaf virus(GFLV) and grapevine leafroll-associated virus Ⅲ(GLRa-3) by PAS-ELISA were studied and an integrated program on detecting GLRaV-3 and GFLV was established. The result of detecting indicated that the outbreak peak of GFLV mainly infecting the upper leaves was in spring and the outbreak peak of GLRaV-3 was in autumn mainly infecting the bottom leaves. |
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| Keywords: | Grape Virus detecting PAS-ELISA Sensitivity GFLV GLRaV-3 |
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