Gliadins as biochemical markers for Aegilops turcomanica genes in wheat lines |
| |
| Authors: | U Vahl T Bringezu G Muller |
| |
| Institution: | Agraroecological Institute e.V. of the Martin Luther University Halle-Wittenberg, Group Biotechnique Bernburg, Strenzfelder Allee 23, D-06406 Bernburg, Germany |
| |
| Abstract: | An Aegilops turcomanica-typical gliadin was discovered in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns of ethanol-soluble seed proteins from wheat lines Tr. 476, 482 and 492, which had been derived from a direct cross of Ae. turcomanica and Triticum aestivum and which revealed powdery mildew resistance due to a putative Ae. turcomanica-introgression. This Ae. turcomanica-derived gliadin was tested for its suitability as biochemical marker. For this purpose, doubled-haploid lines were produced via anther culture from crosses of Tr. 482 and Tr. 492 with actual winter wheat cultivars and breeding lines. Until now, 173 lines with Ae. turcomanica-gene(s) have been selected from 297 doubled haploid wheat lines. |
| |
| Keywords: | Aegilops turcomanica Triticum aestivum alien introgression anther culture biochemical marker doubled-haploid line gliadins powdery mildew resistance SDS-PAGE |
|
|
