2S albumin from buckwheat (Fagopyrum esculentum moench) seeds |
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Authors: | Radovic R S Maksimovic R V Brkljacic M J Varkonji Gasic I E Savic P A |
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Institution: | Faculty of Biology, University of Belgrade, Yugoslavia. |
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Abstract: | Sucrose density gradient centrifugation showed that approximately 30% of total buckwheat proteins migrated with a 2S sedimentation coefficient. The main part of that fraction, polypeptides in the range of molecular mass from 8 to 16 kDa, were water soluble and represented albumins. SDS-PAGE analysis in nonreducing and reducing conditions showed that these polypeptides were not linked by disulfide bonds. The albumins make 25% of total salt soluble proteins, but that content is dramatically reduced under S-deficiency conditions. Determination of amino acid composition showed high methionine (9.2%) and lysine (5.6%) contents. That characteristic offers the possibility of transfer of the genes for individual albumin polypeptides to legumes and cereals limited in those essential amino acids to improve their nutritional quality. |
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