| 葡萄微茎尖培养及葡萄扇叶病毒的ELISA和探针检测 |
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| 引用本文： | 张玉满,田砚亭.葡萄微茎尖培养及葡萄扇叶病毒的ELISA和探针检测[J].北京林业大学学报,1998,20(4):54-58. |
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| 作者姓名： | 张玉满 田砚亭 |
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| 作者单位： | 北京林业大学良种繁育研究中心 |
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| 摘 要： | 在建立‘藤稔’等５个葡萄品种离体培养快速繁殖体系的基础上，通过热处理和微茎尖脱毒培养获得了再生植株；并经过ＰＡＳ－ＥＬＩＳＡ和长臂光敏生物素标记ＧＦＬＶ－ＣＰ基因ｃＤＮＡ探针检测．结果表明白天３８℃夜晚３５℃处理脱除ＧＦＬＶ效果最佳；多次继代培养也有脱除ＧＦＬＶ的作用．从而首次获得了‘藤稔’、‘红地球’、‘９３０７’３个葡萄品种的脱除ＧＦＬＶ的试管苗．ＰＡＳ－ＥＬＩＳＡ与探针平行检测的结果还表明，ＰＡＳ－ＥＬＩＳＡ检测以Ｐ／Ｎ＜１作为阴性标准更为可靠
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| 关 键 词： | 葡萄，热处理，微茎尖培养，GFLV检测，PAS-ELISA，GFLV-CP基因cDNA探针 |
Microapical Culture of Grapevine and Detection of Grapevine Fan Leaf Virus by ELISA and Probe |
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| Abstract: | A rapid propagation in vitro system of 5 grapevine cultivars ‘Teng Ren’etc was established at first,then microapical plantlets were obtained through microapical culture and heat treatment, in which Grapevine Fan Leaf Virus (GFLV)was detected by PAS ELISA and GFLV coat protein(CP) gene cDNA probe labled by long arm photosensitive biotin. The results show that the heat treatment of 38℃(day) and 35℃(night) was more effective to release GFLV, long term tissue culture of more than 20 generations have the effect of releasing GFLV.GFLV free plantlets of ‘Teng Ren ’,‘Red Globe’, ‘9307’have been achieved for the first time. The paralleling detection of ELISA and probe indicated that it is reliable to take P/N<1 as standard of negative sample in PAS ELISA detection. |
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| Keywords: | grapevine heat treatment microapical culture GFLV detect PAS ELISA GFLV CP gene cDNA probe |
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