| 转棉花叶绿体Cu/Zn-SOD基因烟草的获得及其功能的初步验证 |
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| 引用本文： | 马淑娟,喻树迅,范术丽,宋美珍. 转棉花叶绿体Cu/Zn-SOD基因烟草的获得及其功能的初步验证[J]. 分子植物育种, 2007, 5(3): 319-323 |
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| 作者姓名： | 马淑娟 喻树迅 范术丽 宋美珍 |
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| 作者单位： | 中国农业科学院棉花研究所,农业部棉花遗传改良重点实验室,安阳,455000;华中农业大学,武汉,430070;中国农业科学院棉花研究所,农业部棉花遗传改良重点实验室,安阳,455000 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 构建了棉花叶绿体Cu/Zn-SOD基因的植物表达载体,利用农杆菌介导法将其导入NC89烟草,PCR、Southern blotting检测结果显示有4个烟草株系中整合了棉花叶绿体Cu/Zn-SOD基因,SOD酶活性测定结果显示4株转基因烟草植株SOD酶活性都明显高于非转基因烟草,离体叶片暗处理试验结果也证明四株转基因烟草比非转基因烟草SOD酶活下降速度明显减慢,百草枯喷施试验表明,转基因烟草都比非转基因烟草对百草枯的耐性增强,这一系列试验间接地说明外源基因的导入增强了烟草的耐衰老能力,本研究为今后利用SOD基因研究作物的早衰性状奠定了基础.
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| 关 键 词： | 烟草 棉花叶绿体Cu/Zn-SOD基因 农杆菌介导法 SOD酶活性 早衰 |
Development of Transgenic Tobacco with Cotton Chloroplast Cu/Zn-SOD Gene and its Function Identification |
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| Ma Shujuan,Yu Shuxun,Fan Shuli,Song Meizhen. Development of Transgenic Tobacco with Cotton Chloroplast Cu/Zn-SOD Gene and its Function Identification[J]. Molecular Plant Breeding, 2007, 5(3): 319-323 |
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| Authors: | Ma Shujuan Yu Shuxun Fan Shuli Song Meizhen |
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| Abstract: | Plant expression vector of cotton chloroplast Cu/Zn-SOD gene was constructed and introduced into tobacco NC89 via Agrobacterium tumefaciens.The result showed that 4 plantlets display positive reaction in PCR analysis, Southern blotting and SOD enzyme activity detection also indicated that SOD enzyme activity in aparted cotyledon of 4 transgenic plants is higher than that of non transgenic plant, and the sensitivity to paraquat in non transgenic plant is higher than that in 4 transgenic plants. Obviously, the integration of foreign gene increased tobacco resisted caducity activity. |
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| Keywords: | Tobacco Cotton chloroplast Cu/Zn-SOD gene Agrobacterium tumefaciens-mediated SOD enzyme activity Caducity |
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