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白菜型油菜种子芥酸、油酸和硫苷含量的变异及相关性
引用本文:田恩堂,梁海伦,王均军,郭君,曹慧琳,林树春. 白菜型油菜种子芥酸、油酸和硫苷含量的变异及相关性[J]. 贵州农业科学, 2017, 45(1). DOI: 10.3969/j.issn.1001-3601.2017.01.005
作者姓名:田恩堂  梁海伦  王均军  郭君  曹慧琳  林树春
作者单位:贵州大学 油料作物研究所,贵州 贵阳,550025
基金项目:国家自然科学基金项目“QTL作图结合GWAS策略挖掘芥菜型油菜硫代葡萄糖苷代谢的主效调控基因”,贵州省科学技术基金项目“白菜叶片和种子硫代葡萄糖苷主效调控基因的机型定位、挖掘及其代谢机制的解析”,国家教育部留学回国人员科研启动基金项目“甘蓝型油菜硫甙葡萄糖苷调控基因的挖掘及其表达动态分析”,贵州大学博士人才引进基金项目“甘蓝型油菜硫苷含量变异种质筛选及其关联标记开发”,贵州省学位办项目“贵州省作物学省级重点学科建设计划”
摘    要:为筛选及鉴定优异育种资源,为白菜型油菜育种提供基础材料,利用近红外方法测定84份白菜型油菜的芥酸、油酸和硫苷含量,并分析三者之间的相关性。结果表明:芥酸和油酸含量呈极显著负相关,芥酸和硫苷含量呈极显著正相关,油酸和硫苷含量呈极显著负相关;对群体材料进行主成分分析,并提取因子1和因子2进行作图,其中因子1和2分别能解释73.7%和23.2%的表型变异;经聚类分析,有79份材料聚集形成群Ⅰ,另外有5份特殊变异材料偏离群体。白菜型油菜群体的芥酸、油酸和硫苷含量的变异丰富且存在显著相关性,通过聚类分析筛选出5份(32号、45号、46号、50号、59号)优异的白菜型油菜育种材料。

关 键 词:白菜型油菜  芥酸  油酸  硫苷

Variation and Correlation of Erucic Acid,Oleic Acid and Glucosinolate Contents in Brassica rapa Seeds
TIAN Entang,LIANG Hailun,WANG Junjun,GUO Jun,CAO Huilin,LIN Shuchun. Variation and Correlation of Erucic Acid,Oleic Acid and Glucosinolate Contents in Brassica rapa Seeds[J]. Guizhou Agricultural Sciences, 2017, 45(1). DOI: 10.3969/j.issn.1001-3601.2017.01.005
Authors:TIAN Entang  LIANG Hailun  WANG Junjun  GUO Jun  CAO Huilin  LIN Shuchun
Abstract:To screen and identify fine breeding resources,and provide basic materials for B.rapa breeding,near infrared method was employed to determine the contents of erucic acid,oleic acid and glucosinolate,and then the correlation among them were analyzed.Results:Erucic acid content extremely significantly negatively correlated with oleic acid content,while extremely significantly positively correlated with glucosinolate content,and oleic acid content extremely significantly negatively correlated with glucosinolate content.PCA analysis showed that factor 1 and factor 2 could explain 73.7% and 23.2% phenotypic variation,respectively.79 B.rapa accessions clustered into group Ⅰ and the rest five B.rapa accessions (Number 32,45,32,45,46,50 and 59) were scattered.Conclusion:The contents of erucic acid,oleic acid and glucosinolate were of rich variation and significant correlation,five fine B.rapa breeding materials (Number 32,45,32,45,46,50 and 59) were screened through cluster analysis.
Keywords:Brassica rapa  erucic acid  oleic acid  glucosinolate
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