| 基于核酸适配体和阳离子聚合物PAH高效聚集纳米金比色法检测牛奶中四环素 |
| |
| 引用本文： | 罗艳芳,贺兰,詹深山,刘乐,支月娥,周培.基于核酸适配体和阳离子聚合物PAH高效聚集纳米金比色法检测牛奶中四环素[J].上海交通大学学报(农业科学版),2014(6):66-70, 91. |
| |
| 作者姓名： | 罗艳芳 贺兰 詹深山 刘乐 支月娥 周培 |
| |
| 作者单位： | 上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240 |
| |
| 基金项目： | 国家高新技术(863)计划(2012AA101405);公益性行业(农业)科研专项经费项目(200903056);国家自然基金项目(31071860);上海张江国家自主创新示范区专项发展资金重点项目(201209-SJ-A0-001);食品安全工程关键技术及应用服务能力提升(11DZ2210800) |
| |
| 摘 要： | 为满足乳制品中四环素快速检测要求,开发了基于核酸适配体(aptamer)和阳离子聚合物PAH高效聚集纳米金比色检测牛奶中四环素(TET)的新方法。本文优化了PAH和适配体的浓度。最优实验条件下,四环素浓度在一定范围内与A520/A650呈现良好的线性关系,最低检测限(LOD)为95nmol/L,对四环素具有良好的选择性。该方法已成功用于牛奶中四环素的检测,回收率为108%~117%,相对标准偏差为2.9%~3.6%。
|
| 关 键 词： | 四环素 核酸适配体 PAH 纳米金 比色 |
| 收稿时间： | 2013/11/18 0:00:00 |
Colorimetric Detection of Tetracycline in Milk Using Aptamer and Cationic Polymer PAH Efficiently Aggregated Nanogold |
| |
| LUO Yan-fang,HE Lan,ZHAN Shen-shan,LIU Le,ZHI Yue-e and ZHOU Pei.Colorimetric Detection of Tetracycline in Milk Using Aptamer and Cationic Polymer PAH Efficiently Aggregated Nanogold[J].Journal of Shanghai Jiaotong University (Agricultural Science),2014(6):66-70, 91. |
| |
| Authors: | LUO Yan-fang HE Lan ZHAN Shen-shan LIU Le ZHI Yue-e and ZHOU Pei |
| |
| Institution: | School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China and School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China |
| |
| Abstract: | In order to satisfy the requirements of the rapid detection of tetracycline in dairy products,this paper developed a colorimetric method to detect tetracycline using aptamer and cationic polymer PAH efficiently aggregated nanogold.The concentrations of aptamer and PAH were investigated to optimize the reaction conditions.Under the optimization conditions,the concentrations of tetracycline had good linear correlation with A520/A650,and the limit of detection (LOD) was calculated as 95 nmol/L.The method had a good selectivity to tetracycline.The proposed method was successfully applied for the analysis of TET in milk sample with total recoveries ranging from 108% to 117%,and the RSD was 2.9%-3.6%. |
| |
| Keywords: | TET aptamer PAH AuNP colorimetric |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《上海交通大学学报(农业科学版)》浏览原始摘要信息 |
| 点击此处可从《上海交通大学学报(农业科学版)》下载免费的PDF全文 |
|
