| 紫苏EST-SSR分布特征及标记开发 |
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| 引用本文： | 张天缘,宋莉,沈奇.紫苏EST-SSR分布特征及标记开发[J].贵州农业科学,2017,45(9). |
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| 作者姓名： | 张天缘 宋莉 沈奇 |
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| 作者单位： | 1. 贵州大学 生命科学学院/农业生物工程研究院,贵州 贵阳,550025;2. 贵州省农业科学院 油菜研究所,贵州 贵阳,550008 |
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| 基金项目： | 国家自然科学基金项目"紫苏属植物进化与种群结构研究以及贵州紫苏资源遗传多样性评价"，贵州省科技厅农业攻关项目"山地高效紫苏新品种选育及推广"，贵州省科学技术基金项目"贵州紫苏属植物SSR标记开发与资源鉴选" |
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| 摘 要： | 为了解紫苏属植物的遗传多样性,为种质资源的开发利用提供依据,利用软件MISA对从NCBI上获取的5 435条EST序列进行紫苏SSR分析。结果表明:在1 206条EST序列中得到符合条件的SSR为22.19%;共检出1 526个SSR位点,平均覆盖深度为28.87%;EST-SSR有32种重复基元,以二核苷酸重复基元AG/CT最多,占检出总SSR的34.73%;包含低级基元单核苷酸重复、二核苷酸重复和三核苷酸重复,其长度在20bp以上的EST-SSR有386条。最终设计获得包含单核苷酸重复、二核苷酸重复和三核苷酸重复低级基元的SSR引物723条。
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| 关 键 词： | 紫苏 EST-SSR 分子特征 标记开发 |
EST-SSR Distribution Characteristics and Markers Development of Perilla frutescens |
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| ZHANG Tianyuan,SONG Li,SHEN Qi.EST-SSR Distribution Characteristics and Markers Development of Perilla frutescens[J].Guizhou Agricultural Sciences,2017,45(9). |
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| Authors: | ZHANG Tianyuan SONG Li SHEN Qi |
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| Abstract: | To explore the genetic diversity of Perilla, thus to provide references for exploitation and utilization of germplasm resources, a total of 5 435 EST sequences obtained from NCBI by using the software MISA were used to develop SSR markers.Results: Eligible SSRs were sought out in 1 206 EST sequences, with an occurrence frequency of 22.19%.1 526 SSRs were detected and the average coverage depth is 28.87%.The EST-SSR has 32 types of repeat motifs, among which the di-nucleotide repeat motifs of AG/CT has the greatest number, accounting for 34.73% of total SSRs.Through usability evaluation, 386 SSRs containing repeat motifs from one to three were identified in Perilla EST in which the length is more than 20 bp.Through conducting further primer designs on Perilla EST-SSR, 723 pairs of primer with high utilization value were obtained. |
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| Keywords: | Perilla ESS-SSR molecular characteristics marker development |
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