甘蓝型油菜苗期磷高效基因型的TOPSIS法筛选

本研究以8个经田问初步筛选的不同磷营养效率代表品系为材料,在缺磷和施磷条件下进行营养液培养试验,通过测定苗期与磷营养效率相关程度较高的根长、冠部和根部鲜重、单株干重、根冠比、磷含量、冠部和根部酸性磷酸酶活性8个指标,引入TOPSIS法进行综合排序,以筛选出真正的磷高效甘蓝型油菜品系.结果表明:缺磷和施磷条件下,甘蓝型油菜品系W17(第1)和W39(第8)的TOPSIS排名相同,与田间试验结果一致;缺磷处理的W23及施磷处理的W11和W31的TOPSIS排名与田问试验结果一致:缺磷处理的W31和W290及施磷处理的其他4个品系的TOPSIS排名与田间试验结果相差1位;缺磷处理的W11、W27和W288的TOPSIS排名与田问试验结果相差2～4位.可以看出,TOPSIS法准确地筛选出了磷高效基因型甘蓝型油菜W17和磷低效基因型甘蓝型油菜W39;其他品系排序结果也与田问试验比较吻合,表明TOPSIS法可增加材料筛选的准确性,尤其适宜于极端品系的筛选.

Screening phosphorus-efficient genotypes of rapeseed (Brassica napus)at seedling stage by TOPSIS

Based on preliminary screening on the field, eight rapeseed varieties with different phosphorus efficiencies were adopted in the study. Under phosphate (Pi) deficient and sufficient solution culture conditions, eight indices (root length, shoot fresh weight, root fresh weight, single plant dry weight, root to shoot ratio, phosphorus content, shoot APase activity and root APase activity) that are significantly related to phosphorus efficiency in rapeseed seedling were analyzed. The indices were then ranked using TOPSIS method to precisely screen out real phosphorus-efficient rapeseed genotype. The results show that under Pi deficient and sufficient conditions, rapeseed variety, W17 ranks first and W39 ranks last under TOPSIS ranking, which tallies with filed observation. The rankings for W23 under Pi deficient conditions, and W11 and W31 under Pi sufficient conditions also agree with filed observations. But the ranks for W31 and W290 under Pi deficient conditions and the other 4 varieties under Pi sufficient conditions have an order of difference. More significantly, the ranks for W11, W27 and W288 under Pi deficient conditions exhibit 2 ~ 4 orders of difference with filed observations. It then implies that TOPSIS accurately screens out phosphorus-efficient rapeseed genotype W17 and phosphorus-inefficient rapeseed genotype W39. For the other varieties, TOPSIS ranking has a certain degree of coherence with field observations. This indicates that TOPSIS can improve material screening veracity and is especially suitable for screening extreme varieties.